Genome-wide exploration of molecular targets for invasion of malignant pleural mesothelioma cell

对人类恶性间皮瘤（MPM）细胞及其衍生细胞进行全基因组基因表达分析，比较其侵袭能力。在数十个候选基因中，我们重点关注在高侵袭性细胞中普遍下调的基因X。通过敲除基因X，侵袭性加快，但在亲本细胞中的增殖和迁移活性没有改变。在MPM原位植入模型中，X基因敲低组的生存期明显短于抢夺组，提示X基因可能是一种新的治疗MPM侵袭的靶基因。

Genome wide gene expression analysis was performed to compare the human malignant mesothelioma(MPM) cells and their derived cells which had higher invasive capacity. Among the dozens of candidate genes, we focused on gene X, which was commonly down-regulated among highly invasive cells. By knocking down of gene X, invasiveness was accelerated, but proliferation and migration activities were not changed in parental cells. In orthotopic implantation model of MPM, the survival was statistically significantly shorter in gene X knock down group than scramble group, suggesting that gene X might be a target gene for a new treatment against the invasion of MPM.