Identification of "cancer stem cell" of mature lymphoid malignancy

成熟淋巴恶性肿瘤“癌症干细胞”的鉴定

• 批准号: 22700884
• 负责人: ITO Asahi
• 金额: $ 2.5万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700884/
• 关键词: NOGマウス; 幹細胞; がん幹細胞; 悪性リンパ腫

For a search and the identification of malignant lymphoma stem cells, we made the malignant lymphoma model with the NOG mouse using patient samples. The tumor cells of the mantle cell lymphoma invaded the systemic organ including liver, spleen, bone marrow and formed a bulky mass in the abdominal cavity. The cells obtained from the tumor were available for the passage to new NOG mouse. And about the model of the angioimmunoblastic T-cell lymphoma, it was shown that all tumors of the patients and NOG mice from the first to the fourth generation were the same clones as a result of analysis. And B cells, plasma cells and the production of the gamma globulin the reactive component seen with the first generation disappeared the second generation later, and tumor cells were thought to gradually reinforce the tumorigenesity in the NOG mouse in vivo.

为了寻找和鉴定恶性淋巴瘤干细胞，我们利用患者样本用NOG小鼠制作了恶性淋巴瘤模型。套细胞淋巴瘤的肿瘤细胞侵犯肝、脾、骨髓等全身器官，并在腹腔内形成巨大肿块。从肿瘤中获得的细胞可用于传代给新的 NOG 小鼠。而关于血管免疫母细胞性T细胞淋巴瘤模型，分析结果表明，患者和NOG小鼠从第一代到第四代的所有肿瘤都是相同的克隆。第一代中观察到的B细胞、浆细胞和丙种球蛋白的产生（反应性成分）在第二代之后消失，并且肿瘤细胞被认为逐渐增强了NOG小鼠体内的致瘤性。

项目成果

NOGマウスモデルによるT細胞性リンパ腫の病態解明

使用 NOG 小鼠模型阐明 T 细胞淋巴瘤的病理学

• 发表时间: 2011
• 作者: 佐久間英規、稲垣宏;他
• 通讯作者: 他

Establishment of an angioimmunoblastic T-cell lymphoma(AITL) model in the NOG mouse

NOG小鼠血管免疫母细胞性T细胞淋巴瘤（AITL）模型的建立

• 发表时间: 2011
• 作者: 村上雅尚;今城雅之;中島英貴;佐野栄紀;大畑雅典;村上雅尚;村上雅尚;村上雅尚;伊藤旭
• 通讯作者: 伊藤旭