Isolation and characterization of prenyltransferases in furanocoumarin biosynthesis

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In this study, we characterized the biosynthetic gene encoding to the coumarin-specific prenyltransferases to understand the molecular mechanism underlying the biosynthesis of multifunctional and bioactive prenylated coumarins that are produced in plants. We have detected both O-and C-prenyltransferase activities for coumarin substrates in the microsome fraction prepared from lemon(Citrus limon) peel, where large amounts of prenylated coumarins are accumulated. Moreover, we succeeded to isolate the full length of cDNAs, which would be encoding to coumarin-specific prenyltransferases, four genes from Rutaceae and two genes from Apiaceae.