The study of structure and function of protein using wide-q neutron scattering measurement

利用宽q中子散射测量研究蛋白质的结构和功能

• 批准号: 22740280
• 负责人: TAKATA Shinichi
• 金额: $ 1.58万
• 依托单位: Japan Atomic Energy Agency
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22740280/
• 关键词: 生物物理; 小角中性子散乱; 中性子散乱; 小角散乱; 蛋白質

The small and wide angle neutron scattering instrument, TAIKAN, was installed on BL15 in the Materials and Life Science Experimental Facility(MLF) of J-PARC. The beam commissioning was started in January 2012, and Users program was started in March 2012.TAIKAN is designed for efficient measurement in wide-q range of 0.005～ 20[A-1]. 840 3He PSD tubes with 8mm in diameter and 500-1000 mm in effective length were mounted on three detector banks of small-, middle-, and high-angle. In this study, we measured three typical proteins of Myoglobin(Mb),β-Lactoglobulin(LG), and Hemoglobin(Hb) in solution by using TAIKAN, in order to verify the performance of wide-q range. The internal structure of Mb and LG is Helix-rich andβ-sheet-rich, respectively. Hb is composed of 4 sub-units. The sub-unit structure is similar to Mb. According to those three neutron scattering profiles calculated from each atomic coordinate data of Protein Data Bank, they show the drastically difference by the influence of each internal structure within the range of q from 0.2 to 1[A-1]. This indicates the benefit of wide-q measurement to enable the analysis of the intramolecular domain structures and secondary structures.

小型广角中子散射仪TAIKAN安装在J-PARC材料与生命科学实验设施（MLF）的BL15上。光束调试于2012年1月开始，用户计划于2012年3月启动。TAIKAN专为在0.005～20的宽q范围内进行高效测量而设计[A-1]。 840个直径8mm、有效长度500-1000mm的3He PSD管安装在小、中、高角度三个探测器组上。在本研究中，我们使用 TAIKAN 测量了溶液中肌红蛋白 (Mb)、β-乳球蛋白 (LG) 和血红蛋白 (Hb) 三种典型蛋白质，以验证宽 q 范围的性能。 Mb和LG的内部结构分别富含螺旋和β-折叠。 Hb 由 4 个亚单位组成。亚基结构与Mb相似。根据蛋白质数据库各原子坐标数据计算出的这三种中子散射谱，在q从0.2到1的范围内，它们显示出由于各内部结构的影响而产生的巨大差异[A-1]。这表明宽q测量的好处在于能够分析分子内域结构和二级结构。

项目成果

BL15大観の性能およびソフトウエア開発

BL15 Taikan性能和软件开发

• 发表时间: 2012
• 作者: H. Kuwahara;S. Sudo;M. Iijima;and S. Ohya;高田慎一
• 通讯作者: 高田慎一