Development of humanized-liver mice using hepatocyte derived from isolated liver tissue from recipient or donor of living donor liver transplantation

使用来自活体肝移植受者或供者的分离肝组织的肝细胞开发人源化肝小鼠

• 批准号: 23300162
• 负责人: TANOUE Akito
• 金额: $ 5.82万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-11-18 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300162/
• 关键词: 疾患モデル; 手術摘出検体; ヒト肝型マウス; 免疫不全肝障害マウス; 疾患肝細胞; 病態モデル; 肝胆道疾患; 幹細胞移植; 肝細胞移植

In this study, we tried for development of humanized-liver mice in which the liver is reconstitute with human hepatocyte derived from isolated liver tissue from recipient or donor of living donor liver transplantation. We demonstrated that, in identical hepatocyte derived from one recipient, freshly (non-freezing) hepatocytes were most effective engrafted in mouse liver compared with hepatocytes refrigerated at a temperature of 4 degrees overnight or freeze-stocked hepatocytes. On the other hand, there is no correlation between cell viability at isolation of hepatocyte and human albumin level in humanized-liver mice. These results indicated that freshly hepatocyte is useful for development of humanized-liver mice, and improvement of freezing method for hepatocyte may lead to effective development of humanized-liver mice.

在这项研究中，我们尝试开发人性化肝小鼠，其中肝脏是从源自生物供体肝移植的受体或供体的孤立肝组织中的人肝细胞重构的。我们证明，在从一个受体中得出的相同的肝细胞中，新鲜（非冻结）肝细胞最有效地植入了小鼠肝脏中，与在4度的温度下冷藏过夜或冻结的肝细胞相比，肝细胞在小鼠肝脏中。另一方面，在人源化肝小鼠中分离肝细胞时细胞活力与人白蛋白水平之间没有相关性。这些结果表明，新鲜的肝细胞对人性化肝脏小鼠的发展很有用，肝细胞的冻结方法的改善可能会导致人性化肝小鼠的有效发育。

项目成果

HEPATOCYTES TRANSPLANTATION FOR AN INFANT OF ORNITHINE TRANSCARBAMYLASE DEFICIENCY USING CELLS ISOLATED FROM LIVING DONOR REDUCED-GRAFT TISSUE

使用从活体供体减数移植物组织中分离的细胞对鸟氨酸转氨酶缺乏症婴儿进行肝细胞移植

• 发表时间: 2014
• 作者: Matsui A;Horikawa R;Yamamoto A;Sakamoto S;Shigeta T;Nosaka S;Fujimoto J;Tanoue A;Nakamura K;Umezawa A;Matsubara Y;Kasahara M.
• 通讯作者: Kasahara M.

胆道閉鎖症の病因に関する最近の知見-特に発生異常説に関して-

关于胆道闭锁发病机制的最新发现 - 特别是关于异常发育理论 -

• 发表时间: 2011
• 期刊: Organ Biology
• 作者: 中村和昭;田上昭人
• 通讯作者: 田上昭人