Additive effect of rehabilitation after ES cell therapy in neurological disorder model

ES细胞治疗神经障碍模型后康复的累加效应

• 批准号: 23300201
• 负责人: YUGE RUI
• 金额: $ 11.73万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300201/
• 关键词: 微小重力; 細胞塊; 神経再生; 細胞移植; リハビリテーション

Here, we cultured mouse embryonic stem cells (mESCs) in simulated microgravity (SMG) for initial 2 days of neural stem cells (NSCs) induction. Moreover, the cells were transplanted brain injury model mice to examine rehabilitation acted in an additive manner for neurogenesis.In SMG, mESCs formed cell sphere bigger than in 1G condition, and on or after 3 day of induction, NSCs differentiation was enhanced. The cells were activated Notch signal. We consider Notch signal activation is one of the reasons why mESCs can form big cell sphere in SMG to induce NSCs differentiation.The cells were transplanted brain injury model mice. Some mice were treated not only cell therapy but also treadmill exercise for rehabilitation. Functional recovery and neural differentiation were most accelerated and BDNF and GAP43 mRNA expressions increased. We consider rehabilitation acts in an additive manner for reconstruction of neural network through BDNF-GAP43 pathway after cell therapy.

在这里，我们在模拟微重力(SMG)中培养小鼠胚胎干细胞(MESCs)，用于最初2天的神经干细胞(NSCs)诱导。此外，将细胞移植到脑损伤模型小鼠，观察其对神经再生的康复作用。在SMG条件下，mESCs形成的细胞球比1G条件下的大，诱导3d或3d后，NSCs的分化增强。细胞被激活的Notch信号。我们认为Notch信号激活是MESCs在SMG内形成大细胞球诱导NSCs分化的原因之一。一些小鼠不仅接受了细胞治疗，还接受了跑步机锻炼以进行康复。神经功能恢复和神经分化速度最快，BDNF和GAP43mRNA表达增加。我们认为康复在细胞治疗后通过BDNF-GAP43通路重建神经网络具有加性作用。

项目成果

Microgravity Facilitates Stem Cell Proliferation and Neural Differentiation after Cell Transplantation in Neurological Disorder Models.

微重力促进神经疾病模型中细胞移植后的干细胞增殖和神经分化。

• 发表时间: 2012
• 作者: Tanigawa T;Takechi H;Arai H;Yamada M;Nishiguchi S;Aoyama T;Yuge L.
• 通讯作者: Yuge L.

運動が外来性神経幹/前駆細胞に与える影響-脳由来神経栄養因子に着目して-

运动对外源性神经干/祖细胞的影响 - 关注脑源性神经营养因子 -

• 发表时间: 2012
• 作者: 猪村剛史;松本昌也;深澤賢宏;森川久美;孫亜楠;Khalesi Elham;河原裕美;弓削類
• 通讯作者: 弓削類

ヒト骨髄間質細胞の神経分化誘導および移植実験－頭蓋骨と腸骨での比較・検討－

人骨髓基质细胞神经分化诱导及移植实验-颅骨和髂骨的比较研究-

• 发表时间: 2014
• 作者: 大倉優之介;上床裕之;武田正明;籬 拓郎;中田恭輔;深澤賢宏;猪村剛史;Khalesi Elham，古川拓馬;山口 智;河原裕美;栗栖 薫;弓削 類
• 通讯作者: 弓削 類

細胞移植後の運動が神経機能再生へ与える影響

细胞移植后运动对神经功能再生的影响

• 发表时间: 2012
• 作者: 猪村剛史;松本昌也;深澤賢宏;森川久美;孫亜楠;Khalesi Elham;河原裕美;弓削類
• 通讯作者: 弓削類

Electrical stimulation enhanced neurogenin 2 expression of mouse bone marrow stromal cells and intensified the effect of cell transplantation into brain injury.

电刺激增强了小鼠骨髓基质细胞的神经原素2表达，并增强了细胞移植对脑损伤的作用。

• 发表时间: 2012
• 作者: Matsumoto M.;Imura T.;Sun YN.;Fukazawa T.;Morikawa K.;Uwatoko H.;Nakata K.;Khalesi E.;Kawahara Y.;Yuge L.
• 通讯作者: Yuge L.