Additive effect of rehabilitation after ES cell therapy in neurological disorder model

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Here, we cultured mouse embryonic stem cells (mESCs) in simulated microgravity (SMG) for initial 2 days of neural stem cells (NSCs) induction. Moreover, the cells were transplanted brain injury model mice to examine rehabilitation acted in an additive manner for neurogenesis.In SMG, mESCs formed cell sphere bigger than in 1G condition, and on or after 3 day of induction, NSCs differentiation was enhanced. The cells were activated Notch signal. We consider Notch signal activation is one of the reasons why mESCs can form big cell sphere in SMG to induce NSCs differentiation.The cells were transplanted brain injury model mice. Some mice were treated not only cell therapy but also treadmill exercise for rehabilitation. Functional recovery and neural differentiation were most accelerated and BDNF and GAP43 mRNA expressions increased. We consider rehabilitation acts in an additive manner for reconstruction of neural network through BDNF-GAP43 pathway after cell therapy.