Development of in vivo function and safety screening method for food ingrdient

食品原料体内功能及安全性筛选方法开发

• 批准号: 23300279
• 负责人: TOMINAGA NOBUAKI
• 金额: $ 11.9万
• 依托单位: Ariake National College of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300279/
• 关键词: 食品; 生理活性; マイクロアレイ; 機能性; 解析・評価; 生体分子

In this study, we developed a novel evaluation method of functional material and food safety screening using Caenorhabditis elegans as model organism and synthetic medium. We could make a synthetic medium which was made from given ingredients and could cultivate C. elegans from egg to adult. Then we evaluated flavonoids quercetin, rutin which are known as anti-oxidative property. Growth rate of C. elegans was increased by addition of these materials to medium. Demands of trace elements were also determined. Depletion of Copper or Zinc was induced significant loss of fecundity. Moreover we evaluated the effects of insulin and vanadium. The results indicated that the toxicity of vanadium was stimulated by an addition of insulin. We could show that this screening method developed by this study was well work for not only screened for function of food ingredient but also for evaluation of complex effects of food and medicine.

本研究以秀丽线虫为模型生物和合成介质，建立了一种新的功能材料评价方法和食品安全性筛选方法。我们可以制作一种由特定成分制成的合成培养基，可以从卵到成虫培养线虫。然后，我们对被称为抗氧化性能的黄酮类成分--栎素、芦丁进行了评价。在培养基中添加这些物质可以提高线虫的生长速度。还确定了微量元素的需要量。铜或锌的枯竭会导致繁殖力的显著丧失。此外，我们还评估了胰岛素和钒的作用。结果表明，添加胰岛素对钒的毒性有刺激作用。结果表明，本研究建立的筛选方法不仅适用于食品成分的功能筛选，也适用于食品和药物的综合药效评价。

项目成果

バナジウムとインスリンの線虫C. elegansによる複合影響評価

钒和胰岛素对线虫秀丽隐杆线虫的联合作用评价

• 发表时间: 2013
• 作者: 對馬勝年;松山政夫;椛島李歩，冨永伸明，山口明美，中村浩，内田雅也，有薗幸司
• 通讯作者: 椛島李歩，冨永伸明，山口明美，中村浩，内田雅也，有薗幸司

線虫用培地およびそれを用いた被検物質の評価方法

线虫培养基和使用其评价测试物质的方法

• 发表时间: 2013

Effects ofPFOA and fluorotelomer alcohol

PFOA 和氟调聚物醇的影响

• 作者: Tominaga N;Furukawa R;Yamashita S;Yamaguchi A;Arizono K
• 通讯作者: Arizono K

バナジウムの線虫C. elegansによるin vivo評価

线虫对钒的体内评估 秀丽隐杆线虫

• 发表时间: 2013
• 作者: 松山政夫;阿部信介;西田洋子;對馬勝年;椛島李歩，冨永伸明，山口明美，中村浩，内田雅也，有薗幸司
• 通讯作者: 椛島李歩，冨永伸明，山口明美，中村浩，内田雅也，有薗幸司

Comparative study of the biological effects of antimicrobials, triclosan and trichlorcarban, for C. elegans

抗菌药物三氯生和三氯卡班对线虫生物效应的比较研究

• 发表时间: 2014
• 期刊: Journal of Environment and Safety
• 作者: Inokuchi A;Nihira M;Minakoshi M;Yamamoto R;Ishibashi H;Tominaga N;Arizono K
• 通讯作者: Arizono K