High functionality of novel carbonyl sulfide hydrolase aiming at the effective removal of carbonyl sulfide

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Carbonyl sulfide hydrolase (COSase) has been isolated from sulfur oxidizing bacterium. COSase catalyses degradation of carbonyl sulfide (COS) that is a trace constituent in the atmosphere and is considered as one of important gas affecting on the global climate especially on ozone depletion. The aim of our research is to obtain information to make highly efficient enzyme molecule so that powerful removal of COS will be possible. We introduced mutations of several amino acids around the sequence of the active center of the enzyme and alpha 5 helix that is a putative tunnel of substrate or reaction product by forming point mutation and examined the effects against COS degrading activity. Furthermore, we tried to isolate new COS degrading microorganisms from soil samples because soil environment is the major sink of atmospheric COS. Novel COS degrading microorganisms including bacteria and fungi were isolated from the soil.