Biosynthesis of bacterial cellulose utilizing biomass waste as a recycled resource towards a generation of a sustainable society.

利用生物质废物作为再生资源进行细菌纤维素的生物合成，以实现可持续发展的社会。

• 批准号: 14560280
• 负责人: KATAYAMA Yoko
• 金额: $ 2.3万
• 依托单位: National University Corporation Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560280/
• 关键词: Bacterial cellulose; Biomass waste; Recycled resource; Sustainable society; Halotolerant bacteria; Bacteria

We have isolated halotolerant, bacterial cellulose-producing microbes from environments. The purpose of the research project is to reuse salt-containing biomass waste as a recycled resource of cellulose fiber, and also to regenerate high quality paper by adding the fiber. Strain 523 is a halotolerant cellulose producing bacteria that can survive in a medium containing 1.5% NaCl and produced cellulose fiber on the surface of the medium. Although the addition of the bacterial cellulose to the recycled paper is effective for improvement of the quality of the waste paper, as in the case of the cellulose fiber made by the non-halotolerant acetic acid bacteria, cellulose synthesis of strain 523 was lower than acetic acid bacteria. Therefore, in the 2004 fiscal year, culture condition for higher production of the cellulose was examined on the constituent of media, aeration, and other physiochemical conditions. Based on these results, the improved culture which enables high cellulose synthesis was obtained in a liquid culture. Based on the phylogenetic analysis and physiological characteristics, strain 523 was designated as a species in the genus Sinorhizobium.Unlike acetic acid bacteria, Sinorhizobium sp.strain 523 enables to synthesis thinner cellulose fibers in the presence of NaCl. Our results revealed the future application of the cellulose fiber for a possible material and that the generation of a sustainable society by using biomass waste as a recycled resource.

我们从环境中分离出了耐盐、能产生细菌纤维素的微生物。该研究项目的目的是将含盐生物质废弃物作为纤维素纤维的再生资源进行再利用，并通过添加纤维来再生高质量的纸张。菌株523是一种耐盐纤维素生产菌，可以在含有1.5% NaCl的培养基中生存，并在培养基表面产生纤维素纤维。虽然在再生纸中添加细菌纤维素对于提高废纸的质量是有效的，但与非耐盐醋酸菌制造纤维素纤维的情况一样，菌株523的纤维素合成低于醋酸菌。因此，在2004财政年度，对用于提高纤维素产量的培养条件进行了培养基成分、通气和其他理化条件的检查。基于这些结果，在液体培养物中获得了能够实现高纤维素合成的改良培养物。基于系统发育分析和生理特征，菌株523被指定为中华根瘤菌属的一个物种。与醋酸菌不同，中华根瘤菌菌株523能够在氯化钠存在下合成更细的纤维素纤维。我们的研究结果揭示了纤维素纤维作为一种可能材料的未来应用，以及通过使用生物质废物作为回收资源来建立可持续发展的社会。

项目成果

Vertical distribution of carbonyl sulfide observed at Mt.Fuji.

在富士山观察到的硫化羰的垂直分布。

• 发表时间: 2005
• 期刊: Proceedings for The 15^<th> Symposium on Atmospheric Chemistry 15
• 作者: Katayamam Y.

富士山で観測された大気中の硫化カルボニルの鉛直分布

在富士山观测到的大气中硫化羰的垂直分布

• 发表时间: 2006
• 作者: Nagano;K.;et. al.;H. Okochi;H. Okochi;五十嵐 康人;Y. Igarashi;S. Murosaki;K. Miura;目黒亜衣;室崎将史;五十嵐康人;室崎将史;目黒亜衣;五十嵐康人;兼保直樹;高田秀重;Igarashi,Y.;三浦和彦;鈴木一成;Igarashi,Y.;渡辺幸一;鈴木一成;加藤広海
• 通讯作者: 加藤広海

Yamasaki, M.: "Genetic and immunochemical characterization of thiocyanate-degrading bacteria in lake water"Appl.Environ.Microbiol.. 68(2). 942-946 (2002)

Yamasaki, M.：“湖水中硫氰酸盐降解细菌的遗传和免疫化学特征”Appl.Environ.Microbiol.. 68(2)。

Yamasaki, M.: "Effects of anaerobic preculture on aerobic stress responses of Camnvlobacter ieiuni"Biosci.Microflora. 22(1). 21-25 (2003)

Yamasaki, M.：“厌氧预培养对 Camnvlobacter ieiuni 有氧应激反应的影响”Biosci.Microflora。

Yamasaki, M.: "Effects of anaerobic preculture on aerobic stress responses of Campylobacter jejuni."Biosci.Microflora. 22(1). 21-25 (2003)

Yamasaki, M.：“厌氧预培养对空肠弯曲杆菌有氧应激反应的影响。”Biosci.Microflora。