Biosynthesis of bacterial cellulose utilizing biomass waste as a recycled resource towards a generation of a sustainable society.

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We have isolated halotolerant, bacterial cellulose-producing microbes from environments. The purpose of the research project is to reuse salt-containing biomass waste as a recycled resource of cellulose fiber, and also to regenerate high quality paper by adding the fiber. Strain 523 is a halotolerant cellulose producing bacteria that can survive in a medium containing 1.5% NaCl and produced cellulose fiber on the surface of the medium. Although the addition of the bacterial cellulose to the recycled paper is effective for improvement of the quality of the waste paper, as in the case of the cellulose fiber made by the non-halotolerant acetic acid bacteria, cellulose synthesis of strain 523 was lower than acetic acid bacteria. Therefore, in the 2004 fiscal year, culture condition for higher production of the cellulose was examined on the constituent of media, aeration, and other physiochemical conditions. Based on these results, the improved culture which enables high cellulose synthesis was obtained in a liquid culture. Based on the phylogenetic analysis and physiological characteristics, strain 523 was designated as a species in the genus Sinorhizobium.Unlike acetic acid bacteria, Sinorhizobium sp.strain 523 enables to synthesis thinner cellulose fibers in the presence of NaCl. Our results revealed the future application of the cellulose fiber for a possible material and that the generation of a sustainable society by using biomass waste as a recycled resource.