Development of microchip for rapid detection of adipokines and early diagnosis of diabetes mellitus.

血液中的白细胞介素-6和TNF-α通常通过使用96威尔斯孔板的常规夹心ELISA来测量。 然而，传统的检测方法耗时长，不适合快速诊断。 为了克服这些缺点，我们在微芯片上进行了夹心ELISA同时分析。 微芯片由环烯烃共聚物制成，具有4个300 μ m宽和100 μ m深的直微通道。 为了构建IL-6或TNF-α的夹心ELISA，我们使用压电喷墨打印系统将第一抗体沉积和固定在每个微通道的表面上。 该方法准确、灵敏、省时、节省样品和试剂，可用于糖尿病的早期诊断。

Interleukin-6 and TNF-a in the blood are frequently measured by conventional sandwich ELISA using 96 wells plate. However, the conventional assay is time-consuming, and is not suitable for rapid diagnosis. To overcome these drawbacks, we performed a sandwich ELISA on a microchip for simultaneous analysis. The microchip was made of cyclic olefin copolymer with 4 straight microchannels that were 300 um wide and 100 um deep. For the construction of the sandwich ELISA for IL-6 or TNF-a, we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st antibody on the surface of the each microchannel. This microchip-based assay enabled us to determine simultaneously blood IL-6 and TNF-a with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and thus will be applicable to early diagnosis of diabetes mellitus.