Study on functional development, homeostasis and degradation of lubrication mechanism in living tissue using regenerated cartilage model cultured under traction loading

利用牵引负载下培养的再生软骨模型研究活体组织的功能发育、稳态和润滑机制的退化

• 批准号: 23360076
• 负责人: SAWAE Yoshinori
• 金额: $ 12.15万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23360076/
• 关键词: トライボロジー; 細胞・組織; 関節軟骨; 潤滑; 移植・再生医療; 摩擦; メカノトランスダクション

The agarose construct containing freshly isolated primary chondrocytes was prepared as a regenerated cartilage tissue model and cultured under the traction loading applied to the model surface. Then, the effects of the traction loading on the functional development and degradation of lubricious cartilage tissues were examined. Results indicated that the traction loading on the tissue surface could stimulate the biosynthesis of extracellular matrix and bring anisotropic nature in the regenerated cartilage tissue. However, the enhancement in the lubricating ability of cultured tissue surface could not be confirmed experimentally. However, degenerative effects of the excessive shear deformation that would be caused by the disruption of lubrication mechanism could be confirmed, since the cell viability in the agarose construct dropped drastically when the shear strain exceeded 5%.

制备含有新鲜分离的原代软骨细胞的琼脂糖构建体作为再生软骨组织模型，并在施加到模型表面的牵引载荷下培养。然后，检查牵引载荷对润滑软骨组织功能发育和降解的影响。结果表明，施加于组织表面的牵拉载荷可促进再生软骨细胞外基质的合成，使再生软骨组织具有各向异性。然而，在培养的组织表面的润滑能力的增强不能被实验证实。然而，可以证实润滑机制的破坏所引起的过度剪切变形的退化效应，因为当剪切应变超过5%时，琼脂糖构建体中的细胞活力急剧下降。

项目成果

Behavior of Adsorbed Albumin film on CoCrMo Alloy under In-situ observation

CoCrMo合金上吸附白蛋白膜的原位观察行为

• 发表时间: 2013
• 作者: Kazuhiro Nakashima;Yoshinori Sawae;Teruo Murakami;Stefano Mischler
• 通讯作者: Stefano Mischler

Effects of traction loading on regeneration of cartilage tissue in chondrocyte-agarose construct

牵引载荷对软骨细胞-琼脂糖构建体中软骨组织再生的影响

• 作者: Y. Sawae;S. Omata;K. Fukuda;T. Murakami
• 通讯作者: T. Murakami

未使用大腿骨コンポーネントの微小表面突起と超高分子量ポリエチレンとの接触解析

未使用股骨组件的微表面突起与超高分子量聚乙烯之间的接触分析

• 发表时间: 2011
• 期刊: 日本臨床バイオメカニクス学会誌
• 作者: 趙昌熙;他2名
• 通讯作者: 他2名

生体模擬環境における超高分子量ポリエチレン摩耗粉の形態評価

生物模拟环境中超高分子量聚乙烯磨损碎片的形态评价

• 发表时间: 2012
• 作者: Rok Simic;Mitjan Kalin and Tomoko Hirayama;森岡卓志
• 通讯作者: 森岡卓志

Effects of mucosal thickness on the stress distribution and denture stability of mandibular implant-supported overdentures with unsplinted attachments in vitro.

• DOI: 10.4061/2011/894395
• 发表时间: 2011
• 期刊: Journal of dental biomechanics
• 作者: Haruta A;Matsushita Y;Tsukiyama Y;Sawae Y;Sakai N;Koyano K
• 通讯作者: Koyano K