Study on functional development, homeostasis and degradation of lubrication mechanism in living tissue using regenerated cartilage model cultured under traction loading

制备含有新鲜分离的原代软骨细胞的琼脂糖构建体作为再生软骨组织模型，并在模型表面施加牵引载荷下进行培养。然后观察牵引载荷对有色软骨组织功能发育和退化的影响。结果表明，组织表面的牵引载荷可以刺激细胞外基质的生物合成，使再生软骨组织具有各向异性。然而，培养组织表面润滑能力的增强并不能通过实验得到证实。然而，由于剪切应变超过5%，琼脂糖结构中的细胞活力急剧下降，因此可以证实过度剪切变形会导致润滑机制的破坏。

The agarose construct containing freshly isolated primary chondrocytes was prepared as a regenerated cartilage tissue model and cultured under the traction loading applied to the model surface. Then, the effects of the traction loading on the functional development and degradation of lubricious cartilage tissues were examined. Results indicated that the traction loading on the tissue surface could stimulate the biosynthesis of extracellular matrix and bring anisotropic nature in the regenerated cartilage tissue. However, the enhancement in the lubricating ability of cultured tissue surface could not be confirmed experimentally. However, degenerative effects of the excessive shear deformation that would be caused by the disruption of lubrication mechanism could be confirmed, since the cell viability in the agarose construct dropped drastically when the shear strain exceeded 5%.