Integrated understanding of regulations of DNA replication by cell cycle and chromatin

对细胞周期和染色质 DNA 复制调控的综合理解

• 批准号: 23370076
• 负责人: MASUKATA Hisao
• 金额: $ 12.81万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370076/
• 关键词: DNA複製; 細胞周期; クロマチン; テロメア; 分裂酵母; シュゴシン; 複製制御; 染色体; 複製開始; セントロメア; Taz1/TRF1; Sgo2; CDK; CMGヘリカーゼ; Mcm10; replisome; 複製開始点; DNAポリメラーゼイプシロン

We investigated the molecular mechanisms how initiation of DNA replication is regulated by cell cycle and chromatin structures using model organism fission yeast. We found that phosphorylations of multiple replication factors, Sld3 and Drc1, by cell cycle master kinase CDK are crucial to trigger assembly of replication factors at replication origins. We further discovered the role of Mcm10 after assembly of replication factors to initiate actual DNA synthesis. In approach to elucidate the replication timing program, we discovered that telomere-repeat sequence is located near the late-replicating origins and the telomere-binding proteins are involved in replication timing program. These findings are published on high-quality international journals.

我们利用模式生物裂殖酵母研究了细胞周期和染色质结构调控DNA复制起始的分子机制。我们发现，磷酸化的多个复制因子，Sld3和Drc1，由细胞周期主激酶CDK是至关重要的复制因子在复制起点触发装配。我们进一步发现了Mcm10在复制因子组装后启动实际DNA合成的作用。在阐明复制定时程序的方法中，我们发现端粒重复序列位于复制晚期的起点附近，端粒结合蛋白参与了复制定时程序。这些研究结果发表在高质量的国际期刊上。

项目成果

Replication timing program in fission yeast.

裂殖酵母中的复制计时程序。

• 发表时间: 2013
• 作者: Ruan K;Yamamoto TG;Asakawa H;Chikashige Y;Masukata H;Haraguchi T;Hiraoka Y.;Tomoko Nanbu;升方久夫;小川志帆;中村優太;藤保祐樹;升方久夫;升方久夫;升方久夫
• 通讯作者: 升方久夫

Replication timing control by chromatin proteins

通过染色质蛋白控制复制时间

• 发表时间: 2013
• 作者: Oktaviani NA;Pool TJ;Kamikubo H;Slager J;Scheek RM;Kataoka M;Mulder FA;升方久夫
• 通讯作者: 升方久夫

升方研究室

增方实验室

Control of global replication timing by telomere-binding proteins in fission yeast

裂殖酵母中端粒结合蛋白对全局复制时间的控制

• 发表时间: 2012
• 作者: Kanei-Ishii C;Nomura T;Egoh A & Ishii S;升方久夫
• 通讯作者: 升方久夫

Telomere-binding protein Taz1 controls replication timing of the internal late origins in fission yeast

端粒结合蛋白 Taz1 控制裂殖酵母内部晚期起源的复制时间

• 发表时间: 2011
• 作者: 大山良文;和田忠士;古久保哲朗;野崎慎;升方久夫
• 通讯作者: 升方久夫