Studies on molecular assembly of replication proteins and regulation of their functions in initiation of chromosome DNA replication

我们确定了分裂酵母染色体复制起点的全基因组定位，发现着丝粒和交配型位点的异染色质在S期早期复制，而亚端粒的异染色质在S期后期复制。我们发现异染色质蛋白HP1，被认为是DNA复制的负面因素，通过与DNA复制必需的激酶DDK的相互作用，刺激着丝粒和交配型位点的早期S期复制。另一方面，在亚端粒区，端粒结合蛋白在DNA复制的调控中发挥作用。

We determined whole-genome localization of replication origins on fission yeast chromosomes and found that heterochromatin in centromere and mating type locus replicates in early S phase, whereas that in subtelomere replicates late. We discovered that the heterochromatin protein HP1, which was considered as a negative factor to DNA replication, stimulate replication in early S phase at centromere and mating type locus through the interaction with DDK that is an essential kinase for DNA replication. On the other hand, in subtelomeric regions telomere binding proteins play roles in regulation of DNA replication.