Elucidation of diarylheptanpods biosyntheses in achtinorhyzal trees and their actinorhizal symbiosis with Frankia

研究了A.结果表明，该菌对Frankia的侵染率为100%，对Frankia的侵染率为100%。结果表明，A.当根提取物和环状二芳基庚烷类化合物与Frankia一起加入时，促进了sieboldiana幼苗的生长。都是和弗兰克一起加入的通过LC-MS分析证实了根提取物中环状二芳基庚烷类化合物的存在。另一方面，为了鉴定A.结果表明：1.利用下一代测序仪分析了3种植物的总RNA。根据EST序列和cDNA序列，构建了假定的全长ORF。这些序列可能是A. sieboldiana和M. rubra CDS，暂命名为AsCDS和MrCDS。

The influence of the methanol extractives and cyclic diarylheptanoids of A. sieboldiana on Frankia and the seedling were examined. The results indicated that the growth of A. sieboldiana seedling was enhanced when the root extractives and the cyclic diarylheptanoids were added to them with Frankia. were added to them with Frankia. The presence of cyclic diarylheptanoids in the root extractives was confirmed by LC-MS analyses. On the other hand, to identify the cyclic diarylheptanoid synthases (CDS) of A. sieboldiana and Myrica rubra, total RNAs of them were analyzed by next generation sequencer. From the EST sequences and the cDNAs, hypothetical full-length ORFs were constructed. These sequences might be encoding for A. sieboldiana and M. rubra CDS, and they were tentatively named AsCDS and MrCDS, respectively.