Elucidation of diarylheptanpods biosyntheses in achtinorhyzal trees and their actinorhizal symbiosis with Frankia

阐明achtinorhyzal树中二芳基庚烷的生物合成及其与Frankia的放线菌共生

• 批准号: 23380098
• 负责人: KAWAI Shingo
• 金额: $ 12.23万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380098/
• 关键词: アクチノリザル樹木; 環状ジアリールヘプタノイド; フランキア; 窒素固定; ケミカルコミュニケーション; 生合成遺伝子; ジアリールヘプタノイド; 生合成

The influence of the methanol extractives and cyclic diarylheptanoids of A. sieboldiana on Frankia and the seedling were examined. The results indicated that the growth of A. sieboldiana seedling was enhanced when the root extractives and the cyclic diarylheptanoids were added to them with Frankia. were added to them with Frankia. The presence of cyclic diarylheptanoids in the root extractives was confirmed by LC-MS analyses. On the other hand, to identify the cyclic diarylheptanoid synthases (CDS) of A. sieboldiana and Myrica rubra, total RNAs of them were analyzed by next generation sequencer. From the EST sequences and the cDNAs, hypothetical full-length ORFs were constructed. These sequences might be encoding for A. sieboldiana and M. rubra CDS, and they were tentatively named AsCDS and MrCDS, respectively.

研究了A.结果表明，该菌对Frankia的侵染率为100%，对Frankia的侵染率为100%。结果表明，A.当根提取物和环状二芳基庚烷类化合物与Frankia一起加入时，促进了sieboldiana幼苗的生长。都是和弗兰克一起加入的通过LC-MS分析证实了根提取物中环状二芳基庚烷类化合物的存在。另一方面，为了鉴定A.结果表明：1.利用下一代测序仪分析了3种植物的总RNA。根据EST序列和cDNA序列，构建了假定的全长ORF。这些序列可能是A的编码。sieboldiana和M. rubra CDS，暂命名为AsCDS和MrCDS。

项目成果

オオバヤシャブシと放線菌フランキアの共生に関与する抽出成分の検索

寻找参与 S. fasciata 和 Streptomyces Frankiae 共生的提取成分

• 发表时间: 2013
• 作者: 金子貴広;米田夕子;河合真吾;西田友昭;山中高史
• 通讯作者: 山中高史

Nodulation of Alnus japonica and Casuarina equisetifolia in liquid culture after inoculation with Frankia.

液体培养中赤杨和木麻黄接种 Frankia 后结瘤。

• 发表时间: 2013
• 作者: T. Yamanaka;S. Mansour
• 通讯作者: S. Mansour

α-Glucosidase Inhibition Activity by Cyclic Diarylheptanoids from Alnus sieboldiana

• DOI: 10.1080/02773813.2012.723778
• 发表时间: 2013-01-01
• 期刊: JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
• 影响因子: 2
• 作者: Chiba, Koujiro;Ichizawa, Hiroo;Nishida, Tomoaki
• 通讯作者: Nishida, Tomoaki

Cyclic diarylheptanoids may act as signal compounds involved in Alnus sieboldiana-Frankia actinorhizal symbiosis

环状二芳基庚烷类化合物可能作为信号化合物参与桤木-Frankia放线菌共生

• 发表时间: 2014
• 作者: Takahiro Kaneko;Yuko Yoneda;Tomoaki Nishida;Takashi Yamanaka;Shingo Kawai
• 通讯作者: Shingo Kawai

静岡大学農学部森林生物化学研究室

静冈大学农学部森林生物化学实验室