Research on the identification of growth-stimulating peptides from the cerebral ganglion of Pacific abalone

太平洋鲍鱼是东北和北海道地区最重要的养殖品种之一。鲍鱼养殖最关键的问题是其特有的生长缓慢。为了了解鲍鱼的生长调控，我们尝试测定促生长肽。利用总rna构建鲍鱼脑神经节cDNA文库，利用约3300个冒号进行序列分析。至少检测到42个多肽同源物，其中大量克隆为神经肽Y （NPY）同源物。采用cDNA克隆和LC-MS/MS技术分别测定了两个NPY cDNA和肽abNPY-1和2。大型鲍鱼的AbNPY mRNA水平高于小型鲍鱼。注射abNPY后，幼鲍鱼体重增加。这些结果表明，NPY同源肽存在于脑神经节中，该肽参与了太平洋鲍鱼体细胞生长的调控。

Pacific abalone is one of the most important aquaculture species in the Tohoku and Hokkaido areas. The most crucial problem for abalone culture is the characteristically slow growth. In an attempt to understand growth regulation in the abalone, we tried to determined growth-stimulating peptide.cDNA libraries of the cerebral ganglions of abalone were prepared using total RNAs, and about 3300 colons were used for the sequence analysis. At least 42 peptide homologs were detected, and large number of clone is Neuropeptide Y (NPY) homolog. Two NPY cDNAs and peptides, named abNPY-1 and 2, were determined by cDNA cloning and LC-MS/MS.Higher AbNPY mRNA levels were observed in the larger-sized abalone than in those of smaller abalone. Injection of abNPY to juvenile abalone resulted in an increase in body mass. These results suggest that NPY homolog is present in the cerebral ganglion and this peptide is involved in the regulation of somatic growth of the Pacific abalone.