Research on the identification of growth-stimulating peptides from the ovarian envelopes of salmon

大马哈鱼是日本东北和北海道地区最重要的水产资源之一。盐渍鲑鱼卵或伊卡拉具有很高的商业价值，但去除伊卡拉后的卵巢包膜没有太大的实用价值。为了证明卵巢包膜中存在的生长刺激肽及其功能，我们从鲑鱼卵巢包膜中分离出一种参与生长调节的肽，用凝胶过滤色谱法分离卵巢包膜的80%丙酮提取物，并用HPLC法纯化肽。至少纯化了5种肽。这些肽的分子量估计为6.7至17.5 kDa，但这些肽的氨基酸序列未确定。一种10.9 kDa肽刺激虹鳟鱼肝脏胰岛素样生长因子-I mRNA的表达这些结果表明，10.9kDa肽可能参与鲑鱼的生长调节。

Chum salmon is one of the most important aquatic resources in Tohoku and Hokkaido areas of Japan. The salted salmon roe or ikra is high in commercial value, but the ovarian envelopes after removing ikra don't have much utility value. To demonstrate the presence and functions of growth-stimulating peptide in the ovarian envelopes, we tried to identify a peptide involved in growth regulation from ovarian envelopes of salmon.The 80% acetone extracts of the ovarian envelopes was fractionated by gel filtration chromatography, and peptides were purified by HPLC. At least 5 peptides were purified. Molecular weight of these peptides was estimated from 6.7 to 17.5 kDa, but amino acid sequence of these peptides was not determined. A 10.9 kDa peptide stimulates hepatic Insulin-like growth factor-I mRNA expression of rainbow trout. These results suggest that the 10.9kDa peptide might be involved in growth regulation of salmon.