Modification of the fatty acid biosynthetic pathway by transgenesis in a marine teleost

海洋鱼类一般不能产生足够的二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)。因此，有必要在养殖的海洋鱼类饲料中添加鱼油，以供应EPA和DHA。鉴于淡水鱼可以合成EPA和DHA，它们可能表达这一途径所需的所有酶。因此，我们以海水鱼尼伯鱼为材料，建立了一个携带马苏鲑鱼延伸酶基因的转基因品系。脂肪酸分析表明，转基因鱼的肝脏EPA含量较低。然而，转基因鱼中的二十二碳五烯酸含量是非转基因鱼的2.28倍。此外，在转基因鱼中特异性地检测到了四碳五烯酸。因此，我们得出结论，用这些脂肪酸代谢酶转基因鱼类品系的发展可能是操纵鱼类脂肪酸代谢途径的有力工具。

Marine fishes are generally unable to produce sufficient eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is therefore necessary to supplement cultured marine fish species diets with fish oils to supply EPA and DHA. Given that freshwater fishes can synthesize both EPA and DHA, they presumably express all of the enzymes required for this pathway. Therefore, we used marine fish, nibe croaker to produce a transgenic line carrying the elongase gene isolated from masu salmon. Fatty acid analysis revealed that the liver EPA content in the transgenic fish was lower. However, docosapentaenoic acid content in the transgenic fish was 2.28-fold higher than in non-transgenic fish. Further, tetracosapentaenoic acid was specifically detected in the transgenic fish. We therefore conclude that the development of transgenic fish lines with these fatty acid-metabolizing enzymes could be a powerful tool for manipulating fatty acid metabolic pathways in fish.