Modification of the fatty acid biosynthetic pathway by transgenesis in a marine teleost

通过转基因改变海洋硬骨鱼脂肪酸生物合成途径

• 批准号: 23380123
• 负责人: YOSHIZAKI Goro
• 金额: $ 12.06万
• 依托单位: Tokyo University of Marine Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380123/
• 关键词: 脂肪酸代謝酵素; 海産魚; 遺伝子導入; ニベ; 栄養要求; ドコサヘキサエン酸; 脂肪酸代謝; 遺伝子導入魚; ダイオキシン

Marine fishes are generally unable to produce sufficient eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is therefore necessary to supplement cultured marine fish species diets with fish oils to supply EPA and DHA. Given that freshwater fishes can synthesize both EPA and DHA, they presumably express all of the enzymes required for this pathway. Therefore, we used marine fish, nibe croaker to produce a transgenic line carrying the elongase gene isolated from masu salmon. Fatty acid analysis revealed that the liver EPA content in the transgenic fish was lower. However, docosapentaenoic acid content in the transgenic fish was 2.28-fold higher than in non-transgenic fish. Further, tetracosapentaenoic acid was specifically detected in the transgenic fish. We therefore conclude that the development of transgenic fish lines with these fatty acid-metabolizing enzymes could be a powerful tool for manipulating fatty acid metabolic pathways in fish.

海洋鱼类一般不能产生足够的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。因此，有必要在养殖海洋鱼类饲料中补充鱼油，以提供EPA和DHA。鉴于淡水鱼可以合成EPA和DHA，它们可能表达这一途径所需的所有酶。 因此，本研究以海水鱼-日本黄姑鱼为材料，构建了一个转基因株系，该株系携带从日本大麻哈鱼中分离的延伸酶基因。脂肪酸分析表明，转基因鱼肝脏EPA含量较低。然而，转基因鱼中二十二碳五烯酸的含量是非转基因鱼的2.28倍。此外，在转基因鱼中特异性地检测到二十四碳五烯酸。因此，我们的结论是，这些脂肪酸代谢酶的转基因鱼线的发展可能是一个强大的工具，操纵鱼类脂肪酸代谢途径。

项目成果

Establishment of a stable transgenic strain in a pelagic egg spawning marine teleost, Nibe croaker Nibea mitsukurii

• DOI: 10.1016/j.aquaculture.2011.01.042
• 发表时间: 2011-03
• 期刊: Aquaculture
• 影响因子: 4.5
• 作者: Yoji Yamamoto;N. Kabeya;Y. Takeuchi;K. Higuchi;T. Yatabe;K. Tsunemoto;R. Yazawa;Takaichi Kawamura;G. Yoshizaki

アイゴの脂肪酸不飽和化酵素における二重結合導入位置の選択に関わる領域の探索

Aigo脂肪酸去饱和酶中双键引入位置选择涉及的区域的探索

• 发表时间: 2014
• 作者: Naoki Kabeya;Yutaka Takeuchi;Yoji Yamamoto;Ryosuke Yazawa;Yutaka Haga;Shuichi Satoh;Goro Yoshizaki.;矢澤良輔;千葉瑞萌・壁谷尚樹・芳賀穣・佐藤秀一・矢澤良輔・竹内 裕・吉崎悟朗
• 通讯作者: 千葉瑞萌・壁谷尚樹・芳賀穣・佐藤秀一・矢澤良輔・竹内 裕・吉崎悟朗

Modification of EPA/DHA biosynthetic pathway by transgenesis in a marine teleost, nibe croaker.

通过转基因改变海洋硬骨鱼尼贝黄花鱼的 EPA/DHA 生物合成途径。

• 发表时间: 2013
• 作者: Naoki Kabeya;Yutaka Takeuchi;Yoji Yamamoto;Ryosuke Yazawa;Yutaka Haga;Shuichi Satoh;Goro Yoshizaki.
• 通讯作者: Goro Yoshizaki.

Characterization of neuropeptide Y in snakeskin gourami and the change in its expression due to feeding status and melanocortin 4 receptor expression

• DOI: 10.1016/j.ygcen.2012.07.024
• 发表时间: 2012-11-01
• 期刊: GENERAL AND COMPARATIVE ENDOCRINOLOGY
• 影响因子: 2.7
• 作者: Boonanuntanasarn, Surintorn;Jangprai, Araya;Yoshizaki, Goro
• 通讯作者: Yoshizaki, Goro

Modification of fatty acid metabolic pathway by transgenesis in the nibe croaker (Nibea mitsukurii)

通过转基因改变尼伯鱼（Nibea mitsukurii）的脂肪酸代谢途径

• 发表时间: 2012
• 作者: Naoki Kabeya;Yutaka Takeuchi;Yoji Yamamoto;Ryosuke Yazawa;Yutaka Haga;Shuichi Satoh;Goro Yoshizaki.;伊藤智子・芳賀穣・益田玲爾・印出井遥平・大歳光・壁谷尚樹・千葉瑞萌・吉崎悟朗・佐藤秀一;Naoki Kabeya
• 通讯作者: Naoki Kabeya