Development of evaluation method of soil microorganisms evenness

本研究以农田冻融过程中的氧化亚氮排放为研究对象，采用宏基因组和宏转录组技术，从DNA和mRNA水平研究土壤微生物的多样性和均匀性。结果表明，在生物地球化学过程中，物种数量的均匀性比多样性起着重要的作用，表明在生物地球化学过程中，少数物种的数量似乎有所增加。我们应用功能性单细胞分离技术，获得了几个似乎在低温条件下生长并主导该过程的菌株。

We focus on nitrous oxide emission during freeze and thaw process in the field, and to investigate the diversity and evenness of the soil microorganism, metagenomic and metatranscriptomic approaches were applied on DNA and mRNA levels. The results indicate that during the bio-geochemical process, it is rather evenness play an important role than diversity of species number, indicating that few number of species seems to be increased with the process. We have applied the Functional Single Cell separation technique and obtain several isolates which seem to grow and lead the process dominantly under the cold condition.