Development of evaluation method of soil microorganisms evenness

为了研究土壤微生物在冻融过程中的多样性和均匀性，我们在DNA和mRNA水平上应用了宏基因组学和亚转录组学方法。结果表明，在生物地球化学过程中，物种数量的均匀性比多样性起着更重要的作用，表明随着这一过程的增加，物种数量似乎很少。我们应用了功能单细胞分离技术，获得了几个似乎在冷条件下生长并主导过程的分离株。

We focus on nitrous oxide emission during freeze and thaw process in the field, and to investigate the diversity and evenness of the soil microorganism, metagenomic and metatranscriptomic approaches were applied on DNA and mRNA levels. The results indicate that during the bio-geochemical process, it is rather evenness play an important role than diversity of species number, indicating that few number of species seems to be increased with the process. We have applied the Functional Single Cell separation technique and obtain several isolates which seem to grow and lead the process dominantly under the cold condition.