Generation of Monkey/Mouse Chimera with Monkey Liver by Interspecific Blastocyst Injection of Monkey induced Pluripotent Stem Cells

通过种间囊胚注射猴诱导多能干细胞产生具有猴肝的猴/小鼠嵌合体

• 批准号: 23390036
• 负责人: MATSUNAGA Tamihide
• 金额: $ 12.15万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390036/
• 关键词: 胚盤胞補完法; サルiPS細胞; 異種動物間キメラ; ラットiPS細胞; カニクイザル; マーモセット; ラット; tk-NOGマウス; TK-NOGマウス; ES細胞; 薬物動態; FAH

Monkey iPS cell lines were established from dermal fibroblasts by transforming of reprogramming factors. The differentiation ability into triploblastic cells of the iPS cells in vitro was determined by floating cultivation of embryoid bodies. To examine the potential for xenogenic approaches in blastocyst complementation, we injected rat iPS/ES cells into mouse blastocysts generating interspecific chimeras. The interspecific chimeras between mouse and rat using a blastocyst injection technique with rat iPS/ES cells were generated. These data provide basic knowledge of principle for interspecific blastocyst complementation and for generation in vivo of organs derived from monkey iPS/ES cells using a xenogenic environment.

通过重编程因子的转化，从真皮成纤维细胞建立猴iPS细胞系。通过拟胚体漂浮培养法测定iPS细胞体外向三胚层细胞分化的能力。为了研究异种方法在胚泡互补中的潜力，我们将大鼠iPS/ES细胞注射到小鼠胚泡中产生种间嵌合体。采用囊胚注射技术，用大鼠iPS/ES细胞构建了小鼠和大鼠的种间嵌合体。这些数据提供了种间胚泡互补和使用异种环境在体内产生来自猴iPS/ES细胞的器官的原理的基础知识。

项目成果

低分子化合物はヒト人工多能性幹細胞から肝細胞への分化を促進する

低分子化合物促进人诱导多能干细胞分化为肝细胞

• 发表时间: 2012
• 作者: Atsushi Takeda;Masatoshi Nakamura;Hiroaki Fujii;Haruna Tamano;Moto Kajiwara;Motoyoshi Nomizu;近藤祐樹
• 通讯作者: 近藤祐樹

Examination of simple prediction system of the differentiation characteristics into hepatocyte between human induced pluripotent stem cell lines

人诱导多能干细胞系向肝细胞分化特性的简单预测系统的检验

• 发表时间: 2011
• 作者: Daisuke Sato;Nobuhiko Harimoto;Chiho Matsumura;Tamihide Matsunaga
• 通讯作者: Tamihide Matsunaga

再生医療・創薬研究の新規材料として期待されるiPS 細胞

iPS细胞有望成为再生医学和药物发现研究的新材料

• 发表时间: 2013
• 作者: 加茂真宏;岡本良成;大塚雅巳;藤田美歌子;寺坂慎平，前川大志，荒木伸一，田口友彦，新井洋由;松永民秀
• 通讯作者: 松永民秀

Differentiation of Human Induced Pluripotent Stem Cells into Functional Enterocyte-like Cells Using a Simple Method

• DOI: 10.2133/dmpk.dmpk-13-rg-005
• 发表时间: 2014-02-25
• 期刊: DRUG METABOLISM AND PHARMACOKINETICS
• 影响因子: 2.1
• 作者: Iwao, Takahiro;Toyota, Masashi;Matsunaga, Tamihide
• 通讯作者: Matsunaga, Tamihide

Establishment and directed differentiation of induced pluripotent stem cells from glycogen storage disease type Ib patient

• DOI: 10.1111/gtc.12101
• 发表时间: 2013-12-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Satoh, Daisuke;Maeda, Tohru;Matsunaga, Tamihide
• 通讯作者: Matsunaga, Tamihide