Analysis of the roles of thioredoxin peroxidase family for adaptation to oxidative stresses in malaria parasites

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Phenotypes observed in the rodent malaria parasite Plasmodium berghei carrying a targeted knockout of thioredoxin peroxidase-family genes (2-Cys Prx KO and 1-Cys Prx KO) were investigated and have the following results: (1) Expression profiling of Prx mRNAs in 2-Cys Prx KO in the hepatic stage: qRT-PCR analysis revealed overexpression of other Prx mRNAs in the populations, indicating a mechanism underlying the response to oxidative stress in the hepatic-stage. (2) Defect in the hepatic-stage development of 2-Cys Prx KO: IFA analysis displayed smaller sized and fewer merozoite formation in the schizont than the parent strain (WT). (2) Defect in the blood-stage development of 1-Cys Prx KO: mice experiments showed lower parasite density of about 1-5% in the population than WT (>20%) during the first 7 days of infection.