Analysis of the roles of thioredoxin peroxidase family for adaptation to oxidative stresses in malaria parasites

硫氧还蛋白过氧化物酶家族在适应疟疾寄生虫氧化应激中的作用分析

• 批准号: 23390098
• 负责人: KAWAZU Shin-ichiro
• 金额: $ 12.81万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390098/
• 关键词: 感染症; マラリア; ストレス; レドックス; 遺伝子改変原虫

Phenotypes observed in the rodent malaria parasite Plasmodium berghei carrying a targeted knockout of thioredoxin peroxidase-family genes (2-Cys Prx KO and 1-Cys Prx KO) were investigated and have the following results: (1) Expression profiling of Prx mRNAs in 2-Cys Prx KO in the hepatic stage: qRT-PCR analysis revealed overexpression of other Prx mRNAs in the populations, indicating a mechanism underlying the response to oxidative stress in the hepatic-stage. (2) Defect in the hepatic-stage development of 2-Cys Prx KO: IFA analysis displayed smaller sized and fewer merozoite formation in the schizont than the parent strain (WT). (2) Defect in the blood-stage development of 1-Cys Prx KO: mice experiments showed lower parasite density of about 1-5% in the population than WT (>20%) during the first 7 days of infection.

在携带硫氧还蛋白过氧化物酶家族基因靶向敲除的啮齿类疟原虫伯氏疟原虫中观察到的表型（2-Cys Prx KO和1-Cys Prx KO）进行了研究，并具有以下结果：（1）在肝脏阶段2-Cys Prx KO中Prx mRNA的表达谱：qRT-PCR分析揭示了其他Prx mRNA在人群中的过表达，表明肝脏阶段对氧化应激反应的潜在机制。(2)2-Cys Prx KO的肝期发育缺陷：IFA分析显示，与亲本菌株（WT）相比，重组体中的裂殖子尺寸更小，形成的裂殖子更少。(2)1-Cys Prx KO的血液阶段发育缺陷：小鼠实验显示，在感染的前7天，群体中的寄生虫密度比WT（>20%）低约1-5%。

项目成果

Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis

• DOI: 10.1016/j.exppara.2015.04.018
• 发表时间: 2015-07-01
• 期刊: EXPERIMENTAL PARASITOLOGY
• 影响因子: 2.1
• 作者: Hakimi，Hassan;Goto，Yasuyuki;Kawazu，Shin-ichiro
• 通讯作者: Kawazu，Shin-ichiro

Cloning and characterization of Plasmodium vivax thioredoxin peroxidase-1

• DOI: 10.1007/s00436-012-2864-3
• 发表时间: 2012-08-01
• 期刊: PARASITOLOGY RESEARCH
• 影响因子: 2
• 作者: Hakimi, Hassan;Asada, Masahito;Kawazu, Shin-ichiro
• 通讯作者: Kawazu, Shin-ichiro

熱帯熱マラリア原虫の新規転写因子PREBP (Prx regulatory element binding protein)の活性の解析

恶性疟原虫新型转录因子 PREBP（Prx 调节元件结合蛋白）的活性分析

• 作者: 駒木－安田加奈子;奥脇暢;永田恭介;河津信一郎;狩野繁之
• 通讯作者: 狩野繁之

ドキシンペルオキシダーゼ-1遺伝子欠損がPlasmodium berghei肝臓型の発育に及ぼす影響の解析

doxin过氧化物酶1基因缺陷对伯氏疟原虫肝型发育的影响分析

• 发表时间: 2014
• 作者: 薄井美帆;増田-菅沼裕乃;麻田正仁;福本晋也;井上 昇;河津信一郎
• 通讯作者: 河津信一郎

Plasmodium vivax and P. knowlesi: Cloning, expression and functional analysis of peroxiredoxins

间日疟原虫和诺氏疟原虫：过氧化还原蛋白的克隆、表达和功能分析

• 发表时间: 2013
• 作者: Hakimi;H.;and Kawazu;S
• 通讯作者: S