Development of live cell imaging experimental system in babesia parasite

巴贝虫寄生虫活细胞成像实验系统的研制

• 批准号: 24658242
• 负责人: KAWAZU Shin-ichiro
• 金额: $ 2.66万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658242/
• 关键词: 獣医学; 感染症; バベシア; ライブイメージング; 遺伝子改変原虫

The system for stable expression of green fluorescent protein (GFP) in babesia parasite was developed and the GFP-expressing parasite was observed through time-lapse video microscopy. (1) Stable expression of GFP in B. bovis using the WR99210/hDHFR selection system was achieved. The expression cassette was applied for the development of gene targeting (knockout) technique and the knockout parasite was established for the first time. (2) The gliding motility of B. bovis merozoites was revealed for the first time by in vitro time-lapse video microscopy of the GFP-expressing parasite. In vivo infection model of the parasite with SCID mice, with which a part of the circulating erythrocyte had been replaced with bovine cells, was also developed to investigate the gliding motility of the parasite in detail through time-lapse video microscopy.

建立了绿色荧光蛋白（GFP）在巴氏疟原虫中的稳定表达系统，并通过延时视频显微镜观察了表达GFP的巴氏疟原虫。(1)GFP在B中的稳定表达。利用WR 99210/hDHFR选择系统筛选牛的DHFR。将该表达盒应用于基因打靶（敲除）技术的开发，并首次建立了敲除寄生虫。(2)B的滑行运动。首次通过表达GFP的寄生虫的体外延时视频显微镜显示牛裂殖子。在体内感染模型的寄生虫与SCID小鼠，其中一部分循环红细胞已被替换为牛细胞，还开发了详细调查的寄生虫的滑行运动，通过延时视频显微镜。

项目成果

Study on gliding motility of Babesia bovis merozoites using bioimaging analysis.

利用生物成像分析研究牛巴贝虫裂殖子的滑行运动。

• 发表时间: 2014
• 作者: Asada;M.;and Kawazu;S.
• 通讯作者: S.

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Study on gliding motility of Babesia bovis merozo ites using bioimaging analysis

牛巴贝虫早幼虫滑翔运动的生物成像研究

• 发表时间: 2014
• 作者: Asada;M.;and Kawazu;S
• 通讯作者: S

Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy.

• DOI: 10.1371/journal.pone.0035227
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Asada M;Goto Y;Yahata K;Yokoyama N;Kawai S;Inoue N;Kaneko O;Kawazu S
• 通讯作者: Kawazu S

Kawazu Laboratory, NRCPD

NRCPD 河津实验室