Clarifying the pathogenesis of chronic hepatitis C through comprehensive genetic analyses of the virus and host using next-generation sequencer

使用下一代测序仪对病毒和宿主进行全面的遗传分析，阐明慢性丙型肝炎的发病机制

• 批准号: 23390195
• 负责人: ENOMOTO Nobuyuki
• 金额: $ 12.4万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390195/
• 关键词: HCV; HCC; 次世代シークエンス; プロテアーゼ阻害剤; NS5A阻害剤; コア蛋白; ISDR; インターフェロン; リバビリン; 次世代シークエンシング

Hepatitis C virus has been considered to exist in a host as a complex mixture of various viral mutant subpopulations called as "quasispecies". However, analyzing viral quasispecies and its role in the pathogenesis of hepatitis has been difficult due to the technical limitation. In this study, through introducing the revolutionary technology of next-generation sequencing, we disclosed that the quasispecies state and its dynamics had a significant association with antiviral therapies as well as hepatocarcinogenesis.

丙型肝炎病毒被认为作为被称为“准种”的各种病毒突变亚群的复杂混合物存在于宿主中。然而，由于技术限制，分析病毒准种及其在肝炎发病机制中的作用一直很困难。在这项研究中，通过引入下一代测序的革命性技术，我们揭示了准种状态及其动力学与抗病毒治疗以及肝癌发生具有显着相关性。

项目成果

Liver stiffness measured by magnetic resonance elastography as a risk factor for hepatocellular carcinoma: a preliminary case-control study

• DOI: 10.1007/s00330-012-2571-6
• 发表时间: 2013-01-01
• 期刊: EUROPEAN RADIOLOGY
• 影响因子: 5.9
• 作者: Motosugi, Utaroh;Ichikawa, Tomoaki;Araki, Tsutomu
• 通讯作者: Araki, Tsutomu

Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus.

• DOI: 10.1371/journal.pone.0082299
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Shen H;Yamashita A;Nakakoshi M;Yokoe H;Sudo M;Kasai H;Tanaka T;Fujimoto Y;Ikeda M;Kato N;Sakamoto N;Shindo H;Maekawa S;Enomoto N;Tsubuki M;Moriishi K
• 通讯作者: Moriishi K

Changes in plasma vascular endothelial growth factor at 8 weeks after sorafenib administration as predictors of survival for advanced hepatocellular carcinoma.

索拉非尼施用后8周，血浆血管内皮生长因子的变化是晚期肝细胞癌生存的预测指标。

• DOI: 10.1002/cncr.28384
• 发表时间: 2014-01-15
• 期刊: CANCER
• 影响因子: 6.2
• 作者: Tsuchiya, Kaoru;Asahina, Yasuhiro;Matsuda, Shuya;Muraoka, Masaru;Nakata, Toru;Suzuki, Yuichiro;Tamaki, Nobuharu;Yasui, Yutaka;Suzuki, Shoko;Hosokawa, Takanori;Nishimura, Takashi;Ueda, Ken;Kuzuya, Teiji;Nakanishi, Hiroyuki;Itakura, Jun;Takahashi, Yuka;Kurosaki, Masayuki;Enomoto, Nobuyuki;Izumi, Namiki
• 通讯作者: Izumi, Namiki

A new method for induced fit docking (GENIUS) and its application to virtual screening of novel HCV NS3-4A protease inhibitors

诱导拟合对接新方法（GENIUS）及其在新型HCV NS3-4A蛋白酶抑制剂虚拟筛选中的应用

• DOI: 10.1016/j.bmc.2011.09.023
• 发表时间: 2011
• 期刊: Bioorganic & Medicinal Chemistry
• 影响因子: 3.5
• 作者: Takaya D;Sakamoto N;et al.
• 通讯作者: et al.

RETRY Study Group. Retreatment with peginterferon α-2a + ribavirin in patients who failed previous peginterferon α-2b + ribavirin combination therapy

RETRY 研究组。对先前聚乙二醇干扰素 α-2b + 利巴韦林联合治疗失败的患者进行聚乙二醇干扰素 α-2a + 利巴韦林再治疗

• 发表时间: 2012
• 期刊: Dig Dis
• 作者: Ueda T;Tsuchiya K;Hashimoto S;Inoue T;Enomoto N;Inao M;Tanaka A;Kaito M;Imazeki F;Nishiguchi S;Mochida S;Yokosuka O;Yatsuhashi H;Izumi N;Kudo M
• 通讯作者: Kudo M