Development of new anti-tumor therapy for malignant glioma using a drug incorporating polymeric micelle attached with a specific antibody for glioma cancer stem cell

我们建立了恶性胶质瘤细胞皮下移植到裸鼠体内浓缩和富集脑胶质瘤干细胞(GSC)的方法。用这种方法可以获得含有大量GSCs的肿瘤细胞。我们一直在通过一些分子生物学技术来鉴定一种新的针对GSC的分子，然而，我们还没有找到最合适的分子。同时，我们将替莫唑胺和纤维蛋白胶混合，在小鼠模型上研究了该药物的抗肿瘤作用，发现了该药物的有效性，认为应该寻找一种最佳的分子靶向药物。

We developed the method for concentrating and enriching Glioma stem cell(GSC) by subcutaneous implantation of malignant glioma cells into nude mice. By this method we could obtained tumor cells including abundant GSCs. We have been identifying a new molecule specific for GSC by some molecular biological techniques, however, we have not yet found the most suitable molecule. In parallel, we mixed temozolomide and fibrin glue and investigated the anti-tumor effect of this drug in the mouse model.We found the efficacy of this drug and we think we should search for an optimal molecular target drug.