Development of novel nucleic acid medicine artificial bone using bioprocessed NF-kB decoy

利用生物加工的 NF-kB 诱饵开发新型核酸药物人工骨

• 批准号: 23390443
• 负责人: KUBO TAKAYASU
• 金额: $ 12.15万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390443/
• 关键词: NF-κBデコイ; 連通多孔性アパタイト; ESCA; 骨髄由来破骨前駆細胞; TRAP; RANKL; 破骨細胞; インプラントGBR; 骨芽細胞; 骨髄由来破骨前駆細動

This study aimed to develop the newly useful artificial bone material combined the NF-kB decoy which is known as nucleic acid medicine with the polyphosphoric acid reported that is restrained the osteoclasts and promoted the activity of the osteoblasts. It was clarified that the NF-kB decoy was restrained strongly the activity of the osteoclasts in vitro by the TRAP staining and the measurement of TRAP positive cells. It was clarified also that the polyphoshoric acid was promoted the activity of the osteoblast in vitro. Furthermore, it was confirmed that the artificial bone with the polyphosphoric acid and the NF-kB decoy was promoted the bone formation in vivo. In the results of this study, we were able to acquire the significant knowledge about the development of the newly artificial bone which is influenced both of the osteoclasts and the osteoblasts.

本研究旨在开发新型有用的人工骨材料，将被称为核酸药物的NF-kB诱饵与报道的抑制破骨细胞、促进成骨细胞活性的多聚磷酸相结合。通过TRAP染色和TRAP阳性细胞的测定，表明NF-kB诱饵对体外破骨细胞的活性有较强的抑制作用。还表明多磷酸在体外促进成骨细胞的活性。此外，还证实含有多磷酸和NF-kB诱饵的人造骨促进体内骨形成。在这项研究的结果中，我们能够获得有关新人造骨发育的重要知识，该人造骨同时影响破骨细胞和成骨细胞。

项目成果

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

• DOI: 10.4012/dmj.2013-275
• 发表时间: 2014-04-01
• 期刊: DENTAL MATERIALS JOURNAL
• 影响因子: 2.5
• 作者: Doi, Kazuya;Kubo, Takayasu;Akagawa, Yasumasa
• 通讯作者: Akagawa, Yasumasa

Development of composite of poly(P) and bFGF with interconnected porous calcium hydroxyapatite

具有互连多孔羟基磷灰石钙的聚（P）和bFGF复合材料的开发

• 发表时间: 2012
• 作者: 川上滋央;坂本隼一;前田直人;森本雄太;玉田宜之;洲脇道弘;兒玉直紀;沖 和広;皆木省吾;Morita Koji;Morita Koji;前田直人，兒玉直紀，美藤純弘，小橋 基，皆木省吾，松尾龍二;Morita Koji
• 通讯作者: Morita Koji

Histological and mechanical evaluation of the implants placed in the reconstructed bone by interconnected porous calcium hydroxyapatite

通过互连多孔羟基磷灰石钙植入重建骨中的植入物的组织学和机械评估

• 发表时间: 2013
• 作者: Makihara Y;Doi K;Morita K;Kajihara S;Kubo T;Akagawa Y
• 通讯作者: Akagawa Y

連通多孔性ハイドロキシアパタイトによる骨再建部に対するインプラント埋入: 組織学的および力学的評価

使用开孔羟基磷灰石进行骨重建的种植体植入：组织学和力学评估

• 作者: 西田 崇;久保田聡;青山絵理子;ダニーロ ジャヌネ;カレン ライアン;滝川正春;牧原勇介，土井一矢，梶原志穂，久保隆靖，赤川安
• 通讯作者: 牧原勇介，土井一矢，梶原志穂，久保隆靖，赤川安

Polyphosphate-mediated inhibition of tartrate-resistant acid phosphatase and suppression of bone resorption of osteoclasts.

• DOI: 10.1371/journal.pone.0078612
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Harada K;Itoh H;Kawazoe Y;Miyazaki S;Doi K;Kubo T;Akagawa Y;Shiba T
• 通讯作者: Shiba T