Functional analysis of signal transduction system involving membrane transporters in Streptococcus mutans

变形链球菌膜转运蛋白信号转导系统的功能分析

• 批准号: 23390473
• 负责人: NAKANO Michiyo
• 金额: $ 12.31万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390473/
• 关键词: シグナル伝達; バイオフィルム; 齲蝕; ABC膜輸送体; 細菌; ATP結合タンパク; ミュータンスレンサ球菌; 遺伝子解析

Streptococcus mutans metabolizes carbohydrates for adherence to and formation of biofilm on tooth surfaces, which allows the pathogen to tolerate rapid and frequent environmental fluctuations, such as nutrient availability, aerobic-to-anaerobic transitions, and pH changes. Investigation of ammonium transporters is important, as they play crucial roles in the uptake of nutrients by S. mutans in biofilm. The present study focused on characterizing the SMu1510 gene corresponding to nrgA in S. mutans, which is homologous to the ammonium transporter gene in Bacillus subtilis. The growth rate of an nrgA-deficient mutant strain (NRGD) was lower than that of the parental strain in the presence of ammonium. In addition, confocal laser scanning microscopy revealed that the structure of biofilm formed by NRGD was drastically different as compared to formed by the parental strain. These results suggest that the nrgA gene in S. mutans is essential for export of molecules and biofilm formation.

变形链球菌代谢碳水化合物以粘附在牙齿表面并形成生物膜，这使得病原体能够耐受快速和频繁的环境波动，例如营养物可用性、有氧到厌氧的转变和 pH 值变化。铵转运蛋白的研究很重要，因为它们在生物膜中变形链球菌吸收营养物方面发挥着至关重要的作用。本研究的重点是表征变形链球菌中与 nrgA 相对应的 SMu1510 基因，该基因与枯草芽孢杆菌中的铵转运蛋白基因同源。在铵存在下，nrgA 缺陷突变株（NRGD）的生长速率低于亲本菌株。此外，共焦激光扫描显微镜显示，NRGD 形成的生物膜结构与亲本菌株形成的生物膜结构截然不同。这些结果表明变形链球菌中的 nrgA 基因对于分子输出和生物膜形成至关重要。

项目成果

Evaluation of bacterial numbers of Streptococcus mutans in saliva specimens of Japanese children

日本儿童唾液标本中变形链球菌细菌数量的评估

• 发表时间: 2011
• 作者: Fujita K;Matsumoto-Nakano M;Takashima Y;Inagaki S;Nagayama K;Ooshima T
• 通讯作者: Ooshima T

Correlation between cariogenicity and bacteriocin production on Streptococcus mutans clinical isolates

变形链球菌临床分离株致龋性与细菌素产生的相关性

• 发表时间: 2013
• 作者: Matsumoto-Nakano M;Nagayama K;Fujita K;Ooshima T
• 通讯作者: Ooshima T

Streptococcus mutansのプロテオーム解析による表層タンパク発現メカニズムの検討

通过蛋白质组分析研究变形链球菌表面蛋白表达机制

• 作者: 松三友紀;稲垣暁子;藤田一世;仲野道代
• 通讯作者: 仲野道代

Role of ammonium transporter in biofilm formation by Streptococcus mutans.

铵转运蛋白在变形链球菌生物膜形成中的作用。

• 发表时间: 2013
• 作者: Ardin AC;Matsumoto-Nakano M;Nagayama K;Fujita K;Ooshima T.
• 通讯作者: Ooshima T.

Drinking oolong tea inhibits dental plaque accumulation in humans

饮用乌龙茶可抑制人体牙菌斑积聚

• 发表时间: 2011
• 作者: Matsumoto-Nakano M;Fujita K;Inagaki S;Nagayama K;Takashima Y;Kontani M;Y. Kiso Y;Ooshima T
• 通讯作者: Ooshima T