Molecular analysis of gloom-binding Protein in canes Pathogenesis of S. mutans

甘蔗中 gloom 结合蛋白的分子分析 变形链球菌的发病机制

• 批准号: 18592233
• 负责人: NAKANO Michiyo
• 金额: $ 2.45万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592233/
• 关键词: Dental caries; Streptococcus mutans; glucan-binding protein; Molecular microbiology; グルカン結合タンパクB

Streptococcus mutans has been implicated as a major causative agent of dental caries in humans. Bactreial components associated with the adhesion phase of S. mutans include glucosyltransferase, protein antigen c, and proteins that bind glucan. At least four glucan-binding proteins (Gbp) have been identified; GbpA, GbpB, GbpC, and GbpD. In our previous study,the contributions of GbpA and GbpC to the virulence of S. mutans were investigated; however, the biological function of GbpB and its role in the virulence of S. mutans remain to be elucidated. Using a GbpB-deficient mutant strain (BD1), we demonstrated in the present study that GbpB has a role in the biology of S. mutans. The growth rate of BD1 was lower than that of other strains, while it was also shown to be less susceptible to phagocytosis and to form longer chains than the parental strain MT8148. In addition, electron microscope observations of the cell surfaces of BD1 showed that call-wall layers were obscure. These results suggest that GbpB may have an important role in the cell-wall construction and be involved in cell separation and cell maintenance.

变形链球菌是人类龋齿的主要致病因子。与S.变异蛋白包括葡糖基转移酶、蛋白抗原C和结合葡聚糖的蛋白。已经鉴定了至少四种葡聚糖结合蛋白（Gbp）; GbpA、GbpB、GbpC和GbpD。在我们以前的研究中，我们研究了GbpA和GbpC对S. GbpB的生物学功能及其在变形链球菌毒力中的作用尚不清楚。变异体仍有待阐明。利用GbpB缺陷突变株（BD 1），我们在本研究中证明了GbpB在S.变异体BD 1的生长速率低于其他菌株，同时也显示出对吞噬作用不太敏感，并且形成比亲本菌株MT 8148更长的链。此外，电子显微镜观察的细胞表面的BD 1显示调用壁层是模糊的。这些结果表明，GbpB可能在细胞壁的构建中起重要作用，并参与细胞的分离和细胞的维持。

项目成果

Molecular analysis of recombinase A in virulence of Streptococcus mutans

重组酶A对变形链球菌毒力的分子分析

• 发表时间: 2007
• 作者: Matsumoto-Nskano;M;Inagaki;K;Fujita;K;Ooshima;T
• 通讯作者: T

Biologican functions of glucan-binding protein B of Streptococcus mutans.

变形链球菌葡聚糖结合蛋白 B 的生物学功能。

• 发表时间: 2007
• 期刊: Oral Microbiology Immunology 22
• 作者: Fujita K;Matsumoto-Nakano M;Ooshima T.
• 通讯作者: Ooshima T.

Comparison of glucan-binding proteins in cariogenicity of Streptococcus matans

葡聚糖结合蛋白对马坦斯链球菌致龋性的比较

• 发表时间: 2007
• 期刊: Oral Microbiology Immunology 22
• 作者: Matsumoto-Nakano;M;Fujita;K;Ooshima;T
• 通讯作者: T

Role of bacteriocin immunity proteins in the antimicrobial sensitivity of Streptococcus matans.

细菌素免疫蛋白在马坦斯链球菌抗菌敏感性中的作用。

• 发表时间: 2006
• 期刊: Journal of Bacteriology 188
• 作者: Matsumoto-Nakano M;Kuramitsu. HK
• 通讯作者: Kuramitsu. HK

Molecular analysis of recombinase A in virulence of Streptococcus mutans.

重组酶 A 对变形链球菌毒力的分子分析。

• 发表时间: 2007
• 作者: Matsumoto-Nskano M;Inagaki K;Fujita K;Ooshima T
• 通讯作者: Ooshima T