Creation of next generations of periodontal tissue engineering by applying honeycomb microarrays

应用蜂窝微阵列创建下一代牙周组织工程

• 批准号: 23390475
• 负责人: SHIMAUCHI Hidetoshi
• 金额: $ 12.4万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390475/
• 关键词: 歯周外科学; 歯周組織再生; スキャフォールド; トポグラフィー; 歯根膜細胞; ハニカムフィルム; 生体模倣

This research project was aimed to develop the new strategy for periodontal regeneration by combining both technologies of honeycomb microfilm (HF) and conditioning of the extracellular microenvironment for periodontal stem cells. As the result of studies, we indicated that: 1) a biodegradable polymer HF is suitable for the carrier of periodontal ligament cell sheet, 2) autocrine secretion of prostaglandin E2 is essential for the differentiation of periodontal cells stimulated with Ca2+ ion and mechanical stress, and 3) nano-hydroxyapatite could upregulated BMP-2 expression independent of ion release. Furthermore, OCP/ gelatin composite inlay increased angiogenesis as well as bone regeneration in the rat calvaria defect model. Our research results well established the foundations of new concept scaffold technology that can stimulate both cell proliferation and differentiation.

本研究旨在通过结合蜂窝微膜技术和牙周干细胞外微环境的调节，开发牙周再生的新策略。研究结果表明：1）生物可降解聚合物HF适合作为牙周膜细胞片的载体; 2）前列腺素E2的自分泌是Ca ~（2+）和机械应力刺激牙周膜细胞分化所必需的; 3）纳米羟基磷灰石能够上调BMP-2的表达，而不依赖于离子的释放。此外，OCP/明胶复合嵌体在大鼠颅骨缺损模型中增加血管生成以及骨再生。我们的研究结果为新概念支架技术奠定了基础，该技术可以刺激细胞增殖和分化。

项目成果

Interface Oral Health Science 2011, Extracellular phosphate increases bone morphogenic protein-2 expression in human dental pulp cells and human periodontal ligament cells

Interface Oral Health Science 2011，细胞外磷酸盐增加人牙髓细胞和人牙周膜细胞中骨形态发生蛋白 2 的表达

• 发表时间: 2012
• 作者: Nemoto E;Tada H;Shimauchi H
• 通讯作者: Shimauchi H

Morphology and differentiation of human periodontal ligament cells on honeycomb films In Interface Oral Health Science 2011

蜂窝状薄膜上人类牙周膜细胞的形态和分化 In Interface Oral Health Science 2011

• 发表时间: 2012
• 作者: Iwama N;Kawano T;Ishihata H;Shimauchi H;Shimomura M
• 通讯作者: Shimomura M

Calcium-mediated increased expression of fibroblast growth factor-2 acts through NF-kB and PGE2/EP4 receptor signaling pathways in cementoblasts.

钙介导的成纤维细胞生长因子 2 表达增加通过 NF-kB 和 PGE2/EP4 受体信号通路在成牙骨质细胞中发挥作用。

• 发表时间: 2014
• 作者: Sousuke Kanaya ;Eiji Nemoto ;Yukihiko Sakisaka ;and Hidetoshi Shimauchi.
• 通讯作者: and Hidetoshi Shimauchi.

Interface Oral Health Science 2011, Preparation and Biomedical application of selforganized honeycomb-patterned polymer films

Interface Oral Health Science 2011，自组织蜂窝状聚合物薄膜的制备及其生物医学应用

• 发表时间: 2012
• 作者: Kawano T;Iwama N;Ishihata H;Shimauchi H;Shimomura M
• 通讯作者: Shimomura M

Application of honeycomb patterned porous films as functional scaffold for periodontal regenerative therapy

蜂窝状多孔膜作为功能支架在牙周再生治疗中的应用

• 发表时间: 2012
• 作者: Nagayoshi Iwama;Hiroshi Ishihata;Takahito Kawano;Hidetoshi Shimauchi;Masatsugu Shimomura
• 通讯作者: Masatsugu Shimomura