Development of a sophisticated dental implant based on biomimetic engineering

本研究旨在开发一种新的制造方法，可以在种植体固定装置上制造出理想的表面结构，以控制适合骨整合和骨再生的微观表面形貌。为此，我们使用了“微凹压印法”，通过施加蜂窝薄膜。采用湿法腐蚀方法在1型钛板的镜面加工表面制备了规则排列的微坑结构。腐蚀后，我们在钛表面获得了具有相同直径和间距的规则排列的凹坑，作为掩模的蜂窝状网状结构。将人牙周膜（hPDL）或STRO-1^+牙囊（STRO-1^+DF）细胞接种并培养在蜂窝状结构和对照Ti上。hPDL/ STRO-1^+DF细胞在制备的钛表面上表现出刺激的增殖和独特的形态，这与对照钛表面上细胞行为的均匀模式完全不同，这表明我们新开发的微凹压印方法可用于种植体夹具表面的处理和微米级表面形貌的制备。

This study was aimed to develop the new fabrication method that can fabricate an ideal surface structure on implant fixture to control the microscopic surface topography suitable for osseointegration and bone regeneration. For this purpose, we used “micro-dimple imprint method" by applying honeycomb films. We fabricated the regularly arrayed micro-dimple structure on the mirror-like finished surface of Type 1 Ti plate masked with honeycomb film by wet-etching. After the etching, we obtained the regularly arrayed dimples on Ti surface with identical diameter and pitch as a honeycomb mesh structure of the mask. Human periodontal ligament (hPDL) or STRO-1^+dental follicle (STRO-1^+DF) cells were seeded and culture on the honeycomb-fabricated and control Ti. hPDL/ STRO-1^+DF cells showed stimulated proliferation and a unique forms on the fabricated Ti, that is completely different from the uniformed pattern of cell behavior on control Ti surface, suggesting that our newly developed micro-dimple imprint method is useful for the processing of implant fixture surface and fabrication the surface topography at a μm order.