Development of a sophisticated dental implant based on biomimetic engineering

本研究旨在开发一种新的制造方法，可以在种植体支架上制造理想的表面结构，以控制适合骨整合和骨再生的微观表面形貌。为此，我们采用了蜂窝膜的“微凹痕压印法”。采用湿法蚀刻技术，在蜂窝膜掩膜的1型钛板镜面成品表面制备了排列有序的微凹窝结构。刻蚀后，我们在Ti表面得到了具有相同直径和间距的有规则排列的凹窝，作为掩模的蜂窝网结构。将人牙周韧带（hPDL）或STRO-1^+牙滤泡（STRO-1^+DF）细胞接种培养于蜂窝状和对照钛上。hPDL/ STRO-1^+DF细胞在制备的Ti表面表现出受刺激的增殖和独特的形态，这与在对照Ti表面的均匀模式完全不同，这表明我们新开发的微凹窝压印方法可用于植入夹具表面的加工和表面形貌的制备。

This study was aimed to develop the new fabrication method that can fabricate an ideal surface structure on implant fixture to control the microscopic surface topography suitable for osseointegration and bone regeneration. For this purpose, we used “micro-dimple imprint method" by applying honeycomb films. We fabricated the regularly arrayed micro-dimple structure on the mirror-like finished surface of Type 1 Ti plate masked with honeycomb film by wet-etching. After the etching, we obtained the regularly arrayed dimples on Ti surface with identical diameter and pitch as a honeycomb mesh structure of the mask. Human periodontal ligament (hPDL) or STRO-1^+dental follicle (STRO-1^+DF) cells were seeded and culture on the honeycomb-fabricated and control Ti. hPDL/ STRO-1^+DF cells showed stimulated proliferation and a unique forms on the fabricated Ti, that is completely different from the uniformed pattern of cell behavior on control Ti surface, suggesting that our newly developed micro-dimple imprint method is useful for the processing of implant fixture surface and fabrication the surface topography at a μm order.