Development of a sophisticated dental implant based on biomimetic engineering

本研究的目的是开发一种新的制作方法，能够在种植夹具上制造出理想的表面结构，以控制适合骨整合和骨再生的微观表面形貌。为此，我们采用了蜂窝膜的“微窝压印法”。采用湿法腐蚀的方法，在涂有蜂窝膜的TI型钛板镜面加工表面制备了规则排列的微凹坑结构。刻蚀后，我们在钛表面得到了直径和节距相同的规则排列的凹坑，作为掩模的蜂窝网状结构。将人牙周膜细胞(HPDL)或Stro-1^+牙囊细胞(Stro-1^+DF)接种于蜂窝陶瓷和对照钛上。HPDL/Stro-1^+DF细胞在所制备的钛表面表现出刺激增殖和独特的形态，这与对照钛表面均匀的细胞行为模式完全不同，这表明我们新开发的微窝压印方法适用于种植夹具表面的加工和制备μm量级的表面形貌。

This study was aimed to develop the new fabrication method that can fabricate an ideal surface structure on implant fixture to control the microscopic surface topography suitable for osseointegration and bone regeneration. For this purpose, we used “micro-dimple imprint method" by applying honeycomb films. We fabricated the regularly arrayed micro-dimple structure on the mirror-like finished surface of Type 1 Ti plate masked with honeycomb film by wet-etching. After the etching, we obtained the regularly arrayed dimples on Ti surface with identical diameter and pitch as a honeycomb mesh structure of the mask. Human periodontal ligament (hPDL) or STRO-1^+dental follicle (STRO-1^+DF) cells were seeded and culture on the honeycomb-fabricated and control Ti. hPDL/ STRO-1^+DF cells showed stimulated proliferation and a unique forms on the fabricated Ti, that is completely different from the uniformed pattern of cell behavior on control Ti surface, suggesting that our newly developed micro-dimple imprint method is useful for the processing of implant fixture surface and fabrication the surface topography at a μm order.