The field research of atmospheric bioaerosol over the Antarctic: the pioneering ecosystem observation using a tethered balloon

南极地区大气生物气溶胶的研究将成为关注的焦点，因为在南极冰芯中发现微生物，研究大气生物气溶胶的远距离传输是一个值得关注的问题。为了将已有的方法应用于KOSA生物气溶胶的研究，我们将尝试对南极大气生物气溶胶进行直接采样和生物分析。在第54次日本南极研究考察（2012-2013年）期间，使用系留气球在昭和站直接采样了海拔约1，000米的大气生物气溶胶。利用南极海军白濑号破冰船上的生物气溶胶采样装置和雪取谷、Langhovde冰川和Hukuro湾的生物气溶胶采样器对近地面和海面的生物气溶胶进行了采样。我回到日本后，使用下一代测序仪进行了生物分析。

The study of atmospheric bioaerosol over the Antarctic will be focused on because it is attracting attention to find the microorganism in the Antarctic ice cores, investigate the long-range transport of atmospheric bioaerosol. To apply our previous methods in the study on KOSA bioaerosol, the direct sampling and bio-analysis of atmospheric bioaerosol over the Antarctic will be tried. During the 54th Japanese Antarctic Research Expedition (2012-2013), the atmospheric bioaerosol at an altitude of about 1,000 m was directly sampled at Syowa Station using a tethered balloon. The bioaerosols near to the ground and sea surface were sampled by the bioaerosol sampling apparatus on the naval ice-breaker, Shirase, in the Antarctic Ocean and by the bioaerosol sampler at the Yukidori Valley, the Langhovde Glacier, and the Hukuro Cove. After I returned Japan, the bioanalysis using a next generation sequencer was carried out.