Local distribution of cytosolic calcium ions in activated platelets both on collagen fibrils and on Von Willebrand factor under controlled blood flow conditions.

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In order to clarify the initial activation of human platelet in forming thrombi on collagen fibrils or VWF, localizations of intra-cytosolic calcium ion in an individual platelet were imaged in real time using an ultrafast confocal laser microscope. Images of a single platelet were divided into 16 segments (4x4) the intra-cytosolic calcium ion concentrations ([Ca2+]i ) in each segment were measured with software Image J. The [Ca2+]i of individual platelets transiently increased after they first bound to the VWF or the collagen fibrils. The increase in [Ca2+]i started at the center division of each individual platelet under blood flow conditions, although there were no significant difference in [Ca2+]i division in activated platelets. In this study we established the methods of platelet Ca2+ localization under various activated states when platelets bound with VWF or collagen under blood flow conditions.