Development of the method to evaluate the marine ecosystem of mutagen derived from a microbe in the low temperature environment

点击翻译按钮获取中文摘要

We analyzed the synthesis and accumulation of oxidative damage base in the cell in low temperature environment. After isolating deep sea bacteria each from the Boso ( 6,000m) and south mariana area ( 2,900m), and comparing resolution gene MutT of the oxidative damage base with the Escherichia coli origin gene, the specific activity of MutT showed a high value of approximately 2 times than Escherichia coli and the substrate specificity for the oxidation damage base was more remarkable than Escherichia coli and was high. Then, as a result of 8-OH-G quantity in microbe genomic DNA in the deep sea which collected sea water in MR09-04 (JAMSTEC) to check 8-OH-G production, there were more deep sea microbes approximately 10 times than an microbe from surface sea water.