Studies on genes relating to the formation of the testis-ovum in the testis of amphibians by the non-estrogenic action of environmental chemicals

在本研究中，研究了暴露于环境化学物质如双酚A(BPA)、壬基酚(NP)和苯甲酸雌二醇(EB)的两栖动物的睾丸-卵子的形成。首先，对三种两栖动物进行了暴露实验。Rana rugosa蛙的蝌蚪和变态幼蛙的睾丸异常较其他种更为明显。因此，在进一步的实验中，将皱纹夜蛾作为实验动物的模型。接下来，对暴露在环境化学物质中的蝌蚪的睾丸进行组织学分析，以了解睾丸-卵子的形成过程。最后，对暴露于BPA的蝌蚪在睾丸-卵子形成过程中的差异表达基因进行了分析。编码卵黄蛋白原A1和ERRGamma的基因被分离出来，作为与环境化学品的非雌激素作用诱导的睾丸-卵子形成相关的候选基因。

In the present study, the formation of the testis-ovum has been investigated in amphibians exposed to environmental chemicals, such as bisphenol A (BPA), nonylphenol (NP), and estradiol benzoate (EB). Firstly, exposure experiments were performed using three species of amphibians. Abnormal testes were observed more remarkably in the tadpoles and metamorphosed froglets of the frog Rana rugosa than in those of the other species. Thus, R. rugosa was used as a model of an experimental animal in further experiments. Next, the testes were analyzed histologically in the tadpoles exposed to environmental chemicals to know the process of testis-ovum formation. Finally, genes expressed differentially in the tadpoles exposed to BPA were analyzed during the formation of the testis-ova. Genes encoding vitellogenin A1 and ERRgamma were isolated as candidates relating to the formation of testis-ova induced by the non-estrogenic action of the environmental chemical.