Development of a potable ELISA machine using novel antigen-bound polymer

我们开发了一种便携式酶联免疫吸附测定仪的测量内分泌干扰物，壬基酚。将由具有壬基苯基作为侧链的PMMA基聚合物制成的微过滤器设置在垂直堆叠的微反应器中。将微反应器安装在一个25 cm立方体的新开发的自动化流体和检测系统中，并使用该小系统进行竞争ELISA。在0.01-1.00 μ g/l浓度范围内，可建立一条负相关的标准曲线，所得数据的平均变异系数为4.2%。这些结果与商业灵敏分析仪器的灵敏度和重复性相当。

We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.