Development of a potable ELISA machine using novel antigen-bound polymer

点击翻译按钮获取中文摘要

We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.