Development of a potable ELISA machine using novel antigen-bound polymer

我们开发了一种便携式ELISA机，用于测量内分泌干扰物--壬基酚。在竖直堆叠式微反应器中设置了以聚甲基丙烯酸甲酯(PMMA)为基团、以壬基苯基为侧链的微过滤器。将微反置于最新研制的25 cm立方体全自动流控检测系统中，用该系统进行竞争酶联免疫吸附试验。结果表明，在0.01-1.00微克/L浓度范围内，可建立良好的负相关标准曲线，平均变异系数为4.2%。这些结果可与商用灵敏分析仪的灵敏度和重复性相媲美。

We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.