Development of a potable ELISA machine using novel antigen-bound polymer

使用新型抗原结合聚合物开发便携式 ELISA 机器

• 批准号: 23510146
• 负责人: TAKEO MASAHIRO
• 金额: $ 3.58万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23510146/
• 关键词: ELISA; nonylphenol; 分析機; 内分泌撹乱物質; マイクロリアクター; ポータブル分析機; 環境分析; 食品分析; ノニルフェノール; ニトロフェノール; 抗原固定化

We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.

我们开发了一种便携式ELISA机，用于测量内分泌干扰物--壬基酚。在竖直堆叠式微反应器中设置了以聚甲基丙烯酸甲酯(PMMA)为基团、以壬基苯基为侧链的微过滤器。将微反置于最新研制的25 cm立方体全自动流控检测系统中，用该系统进行竞争酶联免疫吸附试验。结果表明，在0.01-1.00微克/L浓度范围内，可建立良好的负相关标准曲线，平均变异系数为4.2%。这些结果可与商用灵敏分析仪的灵敏度和重复性相媲美。

项目成果

Development of a portable fluidic and detection system for ELISA in a 3-dimentional microreactor

三维微反应器中用于 ELISA 的便携式流体和检测系统的开发

• 发表时间: 2013
• 作者: M. Takeo;S. Nii;D.Kato;S. Negoro;S. Yusa;T. Saiki;Y. Takizawa;and Y. Utsumi
• 通讯作者: and Y. Utsumi

Development of a Portable Fluidic and Detection System for ELISA in a 3-Dimensional Microreactor

3 维微反应器中 ELISA 便携式流体和检测系统的开发

• 发表时间: 2013
• 作者: Masahiro Takeo Shoichi Nii;Dai-ichiro Kato;Seiji Negoro;Shinichi Yusa;Tsunemasa Saiki; Yukako Takizawa;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Development of automatic ELISA analytical system for a trace amount of nonylphenol7using a novel antigen-bound microfilter

使用新型抗原结合微过滤器开发痕量壬基酚7自动ELISA分析系统

• 发表时间: 2013
• 作者: M. Takeo;I. Kawaji;A. Nakasuji;T. Tone;Y. Ukita;D. Kato;S. Negoro;S. Yusa;M. Katayama;and Y. Utsumi
• 通讯作者: and Y. Utsumi

Automatic ELISA analytical system for a trace amount of environmental chemicals using a 3-dimensional microreactor with a novel antigen-bound microfilter

使用带有新型抗原结合微过滤器的 3 维微反应器对微量环境化学物质进行自动 ELISA 分析系统

• 发表时间: 2012
• 作者: M. Takeo;I. Kawaji;A. Nakasuji;T. Tone;Y.Ukita;D. Kato;S. Negoro;S. Yusa;M. Katayama;and Y.Utsumi
• 通讯作者: and Y.Utsumi

Three-dimensional Lab-on-CD with enzyme-linkede immunosorbent assay

三维 Lab-on-CD 酶联免疫吸附测定

• 发表时间: 2011
• 作者: T.Azeta;Y.Ukita;M.Takeo;K.Nakagawa;Y.Yusa;and Y.Utsumi
• 通讯作者: and Y.Utsumi