Taxonomic evaluation of actinobacteria isolated from river basin and their preservation as genetic resources

流域放线菌的分类学评价及其遗传资源保存

• 批准号: 23510291
• 负责人: HAYAKAWA Masayuki
• 金额: $ 3.41万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23510291/
• 关键词: 遺伝資源; アクチノバクテリア; 生態学; 分類学; 微生物

Actinobacteria are a group of Gram-positive and high GC content bacteria. The aim of the present study was to establish an effective isolation method for actinobacteria from river basin and evaluate their taxonomic diversity. By using a dilution plating method with 1/5 NBRC 802 medium containing 5% NaCl, a total of 194 actinobacteria were selectively isolated from the river and seashore sediments of Iriomote Island, Okinawa, Japan. According to the 16S rDNA homology search, they classified into 6 families, 10 genera and 23 species. Microbacterium thalassium was recognized as the most predominant species. Of 194 isolates, 48 strains were considered as belonging to new species. These results suggest that the river basin is a promising genetic resource for diverse and novel actinobacteria.

放线菌是一类革兰氏阳性、GC含量高的细菌。建立一种有效的流域放线菌分离方法，并对其分类多样性进行评价。采用稀释平板法从日本冲绳西表岛的河流和海岸沉积物中分离到194株放线菌。根据16S rDNA同源性检索，将它们分为6科10属23种。地中海微杆菌被认为是最主要的菌种。在194个菌株中，有48个菌株被认为是新种。这些结果表明，该流域是一个有前途的遗传资源的多样性和新的放线菌。

项目成果

Janibacter cremeus sp. nov., an actinobacterium isolated from sea sediment.

• DOI: 10.1099/ijs.0.051532-0
• 发表时间: 2013-10
• 期刊: International journal of systematic and evolutionary microbiology
• 影响因子: 2.8
• 作者: Moriyuki Hamada;Chiyo Shibata;T. Tamura;Hideki Yamamura;M. Hayakawa;Ken-ichiro Suzuki

海洋堆積物からのDemequinaceae科放線菌の分離と分類学的研究

海洋沉积物中Demequinaceae放线菌的分离和分类学研究

• 发表时间: 2012
• 作者: 浜田盛之;田村朋彦;山村英樹;早川正幸;鈴木健一朗
• 通讯作者: 鈴木健一朗

Agromyces iriomotensis sp. nov. and Agromyces subtropicus sp. nov., isolated from soil.

• DOI: 10.1099/ijs.0.057349-0
• 发表时间: 2014-03
• 期刊: International journal of systematic and evolutionary microbiology
• 影响因子: 2.8
• 作者: Moriyuki Hamada;Chiyo Shibata;Y. Ishida;T. Tamura;Hideki Yamamura;M. Hayakawa;Ken-ichiro Suzuki

山梨大学生命環境学部生命工学科 早川研究室

山梨大学生命环境科学学院生物技术系早川研究室

Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove.

• DOI: 10.1099/ijs.0.035493-0
• 发表时间: 2012-08
• 期刊: International journal of systematic and evolutionary microbiology
• 影响因子: 2.8
• 作者: Moriyuki Hamada;T. Tamura;Hideki Yamamura;Ken-ichiro Suzuki;M. Hayakawa