Regulation of gvp gene expression in halophilic archaea

古细菌中气泡的形成受环境因素如光、氧分压和培养基中盐浓度的影响。我们的目的是了解在盐生盐杆菌和地中海盐生盐菌的启动子和调节蛋白的水平上，而且在mRNA和翻译的水平上的气泡形成的调节。编码主要结构蛋白GvpA的gvpA基因的启动子被GvpE激活，而GvpD和可能的GvpF似乎参与了阻遏。将详细分析GvpE和GvpD的相互作用。GvpD可能通过蛋白质相互作用和/或磷酸化作用影响GvpE的活性。使用编码盐细菌b-半乳糖苷酶的bgaH阅读框作为报告基因，将在体内研究gvp启动子，以研究TATA盒和用于基础转录的BRE元件的功能以及GvpE介导的激活所需的区域。mcA和pA启动子似乎受到盐的调节，我们将定义所涉及的序列。最后，将研究非翻译mRNA区域（包括发夹环结构）对转录终止和/或转录稳定性的贡献。

The formation of gas vesicles in archaea is influenced by environmental factors such as light, oxygen tension and the salt concentration of the medium. Our aim is to understand the regulation of gas vesicle formation in Halobacterium salinarum and Haloferax mediterranei at the level of the promoters and the regulatory proteins, but also at the level of the mRNA and translation. The promoter of the gvpA gene encoding the major structural protein GvpA that is activated by GvpE, whereas GvpD and possibly GvpF appear to be involved in the repression. The interaction of GvpE and GvpD will be analysed in detail. It is possible that GvpD influences the activity of GvpE by proteinprotein interaction and/or phosphorylation. Using the bgaH reading frame encoding a halobacterial b-galactosidase as reporter, the gvp promoters will be studied in vivo to investigate the functions of the TATA-box and the BRE-element for the basal transcription and also the regions required for the GvpE-mediated activation. The mcA and pA promoters appear to be regulated by salt, and we will define the sequences involved. Finally, the contribution of non-translated mRNAs regions (including hairpin loop structures) to transcript termination and/or transcript stability will be investigated.