The transcription activator GvpE of halophilic Archaea and its interaction partners GvpD, TBP and TFB

两个基因调节器，转录激活因子GvpE，和GvpD表现出抑制功能的气泡形成的卤古菌，将在启动子和转录后水平的行动进行了研究。两个相反方向的启动子PA和PD的gvp基因簇分开的35个核苷酸，和20-nt UAS元件所需的GvpE介导的激活两个启动子重叠与其远端部分的中心。将通过定量实时PCR在野生型和启动子突变体中研究GvpE介导的两种启动子的同时激活。GvpE反应元件位于BRE和TATA盒的旁边，并且GvpE接触TATA盒结合蛋白TBP。我们将研究GvpE是否也接触盐生盐杆菌中的RNA聚合酶或TFB。GvpE和TBP的接触位点，以及GvpE与阻遏蛋白GvpD的相互作用（导致GvpE的降解）将被研究，以获得这些蛋白质的模型：在调节过程中的蛋白质相互作用。我们将监测不同的tfb和tbp基因在不同生长条件下的表达，以确定它们在盐古菌基因表达调控中可能的作用。

The action of two gene regulators, the transcription activator GvpE, and GvpD exhibiting a repressing function during gas vesicle formation of haloarchaea, will be investigated at the promoter and the posttranscriptional level. The two oppositely oriented promoters PA and PD of the gvp gene cluster are separated by 35 nucleotides, and the 20-nt UAS elements required for GvpE-mediated activation of both promoters overlap with their distal portions in the centre. The simultaneous GvpE-mediated activation of both promoters will be studied by quantitative real-time PCR in wild-type and promoter mutants. The GvpE-responsive element locates next to BRE and TATA-box, and GvpE contacts the TATA-box binding protein TBP. We will investigate whether GvpE also contacts the RNA polymerase or TFB in Halobacterium salinarum. The contact sites of GvpE and TBP, and also the interaction of GvpE with the repressor protein GvpD (that leads to the degradation of GvpE) will be investigated to derive a model for these protein:protein interactions during the regulation. We will monitor the expression of the different tfb and tbp genes under different growth conditions to determine their possible role in the regulation of haloarchaeal gene expression.