Molecular identification and characterisation of loci causing heterosis in Arabidopsis thaliana

拟南芥杂种优势位点的分子鉴定和表征

• 批准号: 5403642
• 负责人: Professor Dr. Thomas Altmann
• 金额: --
• 依托单位: Abteilung Molekulare Genetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5403642?language=en
• 关键词: Molecular; identification; characterisation; loci; causing

The proposed project aims at the elucidation of the molecular basis of heterosis (the superior performance of hybrids as compared to their parents) in the plant model system Arabidopsis thaliana. To this end, two divergent accessions, C24 and Col, will be studied, the F1 hybrids of which were shown to exhibit hybrid vigour. Through the analysis of the two parent lines, their F1 hybrids, ca. 400 derived genotyped recombinant inbred lines (RILs) and their testcross hybrids, heterotic quantitative trait loci (hQTL) will be identified that affect growth (biomass, leaf area) at early stages of plant development. Gene expression profiling experiments will be performed in this program (such as the analysis of biomass and leaf area at later stages of development, protein profiles, metabolite profiles and physiological and developmental processes during seedling etablishmend) that use the same genetic material in order to gain in-depth insight into the molecular processes associated with heterosis.

拟议的项目旨在阐明植物模式系统拟南芥中杂种优势的分子基础(杂交种的表现优于其双亲)。为此，将对两个不同的材料C24和Col进行研究，其F1杂种表现出杂交优势。通过对两个亲本及其F_1代、约400个衍生的遗传型重组自交系及其测交杂交种的分析，将确定在植株发育早期影响生长(生物量、叶面积)的杂种数量性状基因座(HQTL)。本项目将使用相同的遗传物质进行基因表达谱实验(如分析幼苗发育后期的生物量和叶面积、蛋白质谱、代谢物谱以及生理和发育过程)，以深入了解与杂种优势相关的分子过程。