Functional analysis of subtilisin-like serine proteases in Arabidopsis thaliana

拟南芥枯草杆菌蛋白酶样丝氨酸蛋白酶的功能分析

• 批准号: 5352164
• 负责人: Professor Dr. Thomas Altmann
• 金额: --
• 依托单位: Lancaster University
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5352164?language=en
• 关键词: Functional; analysis; subtilisin; like; serine

The proposed project aims at the functional analysis of the family of subtilisin-like serin protease genes in Arabidopsis thaliana, which includes about 50 members. Known eukaryotic subtilases (mammalian in particular), activate through specific cleavage prohormones or proreceptors involved in the control of various developmental or metabolic processes. Several plant subtilases have been isolated that are potentially involved in signalling processes triggered by environmental stimuli (wounding, pathogen attack) or related to developmental processes. Through the analysis of a corresponding mutant, the Arabidopsis SDD1 subtilase was shown to fulfill an essential function in the control of stomatal density and distribution. To gain insight into the roles of Arabidopsis subtilase genes, they will be subjected to a functional genomics program including sequence analysis, expression studies, and mutant identification and characterisation.

拟开展的项目旨在对拟南芥中的类枯草杆菌蛋白酶丝氨酸蛋白酶基因家族进行功能分析，该家族约有50个成员。已知的真核枯草杆菌酶（特别是哺乳动物）通过参与控制各种发育或代谢过程的特异性切割激素原或受体原来激活。已经分离出几种植物枯草杆菌酶，其潜在地参与由环境刺激（创伤、病原体攻击）触发的信号传导过程或与发育过程相关。通过对相应突变体的分析，拟南芥SDD1枯草杆菌酶被证明在气孔密度和分布的控制中发挥重要作用。为了深入了解拟南芥枯草杆菌酶基因的作用，他们将进行功能基因组学计划，包括序列分析，表达研究，突变体的鉴定和表征。