Heterosis-associated gene expression in early development

早期发育中杂种优势相关基因的表达

• 批准号: 5403666
• 负责人: Professor Dr. Stefan Scholten
• 金额: --
• 依托单位: Abteilung Nutzpflanzengenetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5403666?language=en
• 关键词: Heterosis; associated; gene; expression; early

There is emerging evidence that differential gene expression between hybrids and their inbred parents is associated with heterosis and that these altered expression pattern are connected with allele-specific silencing. An important step towards the understanding of this phenomenon would be the knowledge which genes facilitate the identification of differential expressed genes. The first step will be the construction of glass microarrays with cDNAs enriched for differential expressed genes between hybrids and the corresponding inbred lines by supression substractive hybridization. Because expression patterns are established early after fertilization cDNAs representing active genes or stored transcripts of female gametes will be included in these arrays. For expression profiling of hybrids and their inbred parents unigene microarrays will be used in addition to comprehend expression data of as much genes as Early stages of embryo and endosperm development, a few days after fertilization, will be microdissected and used for comparative microarray hybridization between hybrids and the corresponding inbred parents. Comparison of reciprocal F1 hybrids will provide a system to identify genes associated with parentof-origin effects. Very early developmental stages will be profiled to facilitate the identification of genes involved in cell fate determination and constitution of differential expression patterns. Additional profiling of non-pollinated ovules will integrate the mother tissue to provide a more comprehensive view on gene expression related to grain These expression profiles will serve as a basis to clone genes for functional analysis and exploration of gene regulatory networks associated with heterosis.

有新的证据表明，杂种和它们的近交亲本之间的差异基因表达与杂种优势有关，并且这些改变的表达模式与等位基因特异性沉默有关。理解这种现象的重要一步是了解哪些基因有助于识别差异表达基因。第一步是利用抑制差减杂交技术富集杂交种与相应自交系之间差异表达基因的cDNA，构建玻璃微阵列。因为表达模式在受精后早期建立，所以代表活性基因的cDNA或雌性配子的储存转录本将包括在这些阵列中。对于杂种及其近交亲本的表达谱分析，除了理解尽可能多的基因的表达数据之外，还将使用单基因微阵列。在受精后几天，将对胚胎和胚乳发育的早期阶段进行显微解剖，并用于杂种与相应的近交亲本之间的比较微阵列杂交。正反交F1杂种的比较将提供一个系统，以确定与父母的起源效应相关的基因。非常早期的发育阶段将进行分析，以促进参与细胞命运决定和差异表达模式的构成的基因的鉴定。非授粉胚珠的额外分析将整合母体组织，以提供与谷物相关的基因表达的更全面的视图。这些表达谱将作为克隆基因的基础，用于功能分析和探索与杂种优势相关的基因调控网络。