Heterosis-associated gene expression in early development

早期发育中杂种优势相关基因的表达

• 批准号: 5403666
• 负责人: Professor Dr. Stefan Scholten
• 金额: --
• 依托单位: Abteilung Nutzpflanzengenetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5403666?language=en
• 关键词: Heterosis; associated; gene; expression; early

There is emerging evidence that differential gene expression between hybrids and their inbred parents is associated with heterosis and that these altered expression pattern are connected with allele-specific silencing. An important step towards the understanding of this phenomenon would be the knowledge which genes facilitate the identification of differential expressed genes. The first step will be the construction of glass microarrays with cDNAs enriched for differential expressed genes between hybrids and the corresponding inbred lines by supression substractive hybridization. Because expression patterns are established early after fertilization cDNAs representing active genes or stored transcripts of female gametes will be included in these arrays. For expression profiling of hybrids and their inbred parents unigene microarrays will be used in addition to comprehend expression data of as much genes as Early stages of embryo and endosperm development, a few days after fertilization, will be microdissected and used for comparative microarray hybridization between hybrids and the corresponding inbred parents. Comparison of reciprocal F1 hybrids will provide a system to identify genes associated with parentof-origin effects. Very early developmental stages will be profiled to facilitate the identification of genes involved in cell fate determination and constitution of differential expression patterns. Additional profiling of non-pollinated ovules will integrate the mother tissue to provide a more comprehensive view on gene expression related to grain These expression profiles will serve as a basis to clone genes for functional analysis and exploration of gene regulatory networks associated with heterosis.

有新兴的证据表明，杂种及其近交的父母之间的差异基因表达与杂种有关，并且这些改变的表达模式与等位基因特异性的沉默有关。朝着理解这种现象的重要一步是基因促进差异表达基因的知识。第一步将是通过通过替补丝杂交构建杂种和相应的杂交线之间的差异表达基因的玻璃微阵列的构建。因为表达模式是在代表活跃基因或女配子的储存的cDNA后早期建立的，这些阵列将包括在这些阵列中。为了表达杂种及其近交父母的表达谱图，除了了解胚胎和胚乳发育的早期阶段的表达数据外，还将使用施肥后几天的表达数据进行微解析和使用，并将其用于杂交和相应的杂交父母之间的比较微阵列杂交。互惠F1杂种的比较将提供一个系统来识别与亲素 - 源性作用相关的基因。非常早期的发育阶段将进行介绍，以促进鉴定涉及细胞命运的基因确定和差异表达模式的构成。非花授粉胚珠的额外分析将整合母组织，以对与谷物相关的基因表达提供更全面的看法，这些表达谱将作为克隆基因的基础，用于功能分析和探索与杂种相关的基因调节网络。