Biomolecular recognition ability of RecA as a model protein studied by using carbon nanotubes

利用碳纳米管研究RecA作为模型蛋白的生物分子识别能力

• 批准号: 23540479
• 负责人: UMEMURA KAZUO
• 金额: $ 3.41万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23540479/
• 关键词: 分子認識; RecA; DNA; カーボンナノチューブ; 原子間力顕微鏡; 電気泳動; 蛋白質; 生体分子認識

RecA proteins and single-stranded DNA binding (SSB) proteins were reacted with hybrids of DNA and carbon nanotubes (CNT) in order to verify biomolecular recognition ability of the protein molecules. Hybrids of DNA and CNT were assumed as dummy molecules of DNA. We found that RecA molecules could bind onto DNA molecules adsorbed on CNT surfaces. However, helical filamentous structures that are formed on bare DNA molecules were not appeared in the case of the hybrids of DNA and CNT. We also found that SSB proteins could distinguish single-stranded DNA from double-stranded DNA molecules even on CNT surface. These results basically provided helpful information to understand tolerance of biomolecular recognition ability of the proteins. Furthermore, our data is valuable to evaluate conditions of DNA molecules on CNT surfaces.

将RecA蛋白和单链DNA结合蛋白(SSB)分别与DNA和碳纳米管(CNT)杂交物反应，以验证蛋白质分子的生物分子识别能力。DNA和碳纳米管的杂交体被认为是DNA的假分子。我们发现RecA分子可以结合到吸附在CNT表面的DNA分子上。然而，在DNA和CNT杂交物的情况下，在裸露的DNA分子上形成的螺旋丝状结构没有出现。我们还发现，即使在碳纳米管表面，SSB蛋白也能区分单链和双链DNA分子。这些结果基本上为了解蛋白质的生物分子识别能力的耐受性提供了有用的信息。此外，我们的数据对于评估DNA分子在碳纳米管表面的状态是有价值的。

项目成果

Atomic force microscopy study of DNA-wrapped single-walled carbon nanotubes in aqueous conditions

水性条件下 DNA 包裹的单壁碳纳米管的原子力显微镜研究

• 发表时间: 2012
• 作者: Takuya Hayashida;Kazuo Umemura
• 通讯作者: Kazuo Umemura

Nanoscopic characterization of DNA molecules adsorbed on carbon nanotubes

碳纳米管上吸附的 DNA 分子的纳米表征

• 发表时间: 2014
• 作者: Nishizawa;M.; Urano;A.; Fujimori;H.;Kazuo Umemura
• 通讯作者: Kazuo Umemura

Interaction of Biomolecules and Carbon Nanotubes Studied by Atomic Force Microscopy

用原子力显微镜研究生物分子和碳纳米管的相互作用

• 发表时间: 2013
• 作者: K. Tabayashi;O. Takahashi (他8名);Kazuo Umemura
• 通讯作者: Kazuo Umemura

Structures of Hybrids of DNA and Carbon Nanotubes Observed by Atomic Force Microscopy in Air and in Liquids

用原子力显微镜在空气和液体中观察到 DNA 和碳纳米管杂化物的结构

• 发表时间: 2014
• 期刊: Journal of Multifunctional Composites
• 作者: Kazuo Umemura;Takuya Hayashida;Daisuke Nii;Yuuki Ymaguchi;Takuya Kawashima
• 通讯作者: Takuya Kawashima

AFM画像の連続断面解析によるDNA-カーボンナノチューブ複合体の表面形状評価

通过 AFM 图像的连续横截面分析评估 DNA-碳纳米管复合材料的表面形状

• 发表时间: 2012
• 作者: 平田知宏;林田拓也;梅村和夫
• 通讯作者: 梅村和夫