Development of pH sensitive liposome for organic photovoltaics with fullerene

虽然脂质体作为一种药物和基因的载体已被广泛研究，但在脂质体包涵体的释放方面仍存在一些问题。为了在细胞中释放药物，脂质体必须在酸性环境中不稳定，例如在内吞囊泡内。在本研究中，我们合成了烷基链上含有二硫基团的磷脂，并利用含有磷脂的二硫基团制备了ph敏感脂质体。在N，N'-二异丙基碳二亚胺和4-二甲氨基吡啶存在下，以sn-甘油-3-磷胆碱和含脂肪酸二硫基为原料合成了该磷脂。在pH7.4条件下制备了pH敏感脂质体，并研究了脂质体的pH依赖性不稳定性。将ph敏感脂质体浇铸在用于有机光伏的ITO电极上，并与酸进行酯化。酸处理改变了脂质体的形态。

Although liposome has been studied as a career of drug and gene delivery, there are some problems in the release of inclusions of liposome. In order to release drugs in a cell, liposome should be destabilized in acid enviroments such as inside of endocytic vesicles. In this study, we synthesized the phospholipids with disulfide groups in alkyl chains, and prepared pH-sensitive liposome using disulfide groups containing phospholipids. The phospholipid was synthesized from sn-glycero-3-phosphocholine and disulfide group containing fatty acid in the presence of N,N'-diisopropylcarbodiimide and 4-dimethylaminopyridine. The pH-sensitive liposome was prepared at pH7.4, and pH dependent destabilization of the liposome was investigated. pH-sensitive liposome was casted on ITO electrode which was used for organic photovoltaic, and terated with acid. The morphology of the liposome was changed by the treatment of acid.