Studies on a blue-light sensing system of fern Adiantum PHY3 photoreceptor

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Adiantum photoreceptor PHY3 acts as both a phytochrome and a phototropin. PHY3 includes "Photosensory Core Module" (PCM) of phytochromes and PHY3 PCM could perceive not only red- but also blue-light signals. To study blue-light sensing molecular mechanisms of PHY3 PCM, I substituted PHY3 PCM to Arabidopsis phytochrome PCMs. These chimeric photoreceptors can induce blue-light dependent hypocotyl phototropic response, however, no significant red-light induced hypocotyl bending toward the light source was observed. Hence, PHY3 PCM could not be interchangeable to a corresponding region of either phyA or phyB of Arabidopsis. I compared PHY3 PCM amino acid sequence to other phytochrome PCMs and found the PHY3 specific gaps in PCM region. These results suggest that such a PHY3 characteristic gaps in PCM could confer blue-light sensing ability to PHY3 PCM region.