Functional analyses of fern photoreceptor PHY3

蕨类植物光感受器 PHY3 的功能分析

• 批准号: 17570041
• 负责人: KANEGAE Takeshi
• 金额: $ 2.24万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570041/
• 关键词: plant; development; differentiation; photoreceptor; signal transduction; environmental response; フィトクロム; フォトトロピン

Phytochrome 3 (PHY3) is a red-light receptor found in some ferns, which preferentially grow under weak light conditions. Based on its predicted domain organization, fern PHY3 looks like a chimeric protein consists of both red・and blue-light photoreceptors. N-terminal half of PHY3 contains a chromophore-binding domain of phytochrome and its C-terminal half shows a remarkable similarity to phototropin, containing two LOV domains and a Ser/Thr kinase domain. To study functions of PHY3 polypeptide, we introduced CaMV 35S promoter driven Adiantum PHY3 gene into Arabidopsis phot1-5 phot2-1 plant and observed the phototropic responses. Here we report that PHY3 acts as a dual-channel photoreceptor with both the red-light sensing system of phytochrome and the blue-light sensing system of phototropin. Although these sensing-systems usually reside in independent photoreceptors, these two different light-sensing systems cooperate and generate a synergistic effect within PHY3 molecule, and consequently confer the enhanced light-sensitivity on PHY3.

植物色素3（Phy3）是在某些蕨类植物中发现的红光受体，在弱光条件下优先生长。根据其预测的域组织，Fern Phy3看起来像是嵌合蛋白由红色和蓝光光感受器组成。 phy3的N末端一半包含植物色素的发色团结合结构域，其C末端一半表现出与光子蛋白的显着相似性，其中包含两个LOV结构域和一个Ser/Thr激酶结构域。为了研究Phy3多肽的功能，我们将CAMV 35S启动子驱动的adiantum Phy3基因引入了拟南芥照片1-5 Photo2-1植物，并观察到了光子反应。在这里，我们报告说，Phy3充当双通道光感受器，具有植物色素的红光灵敏度系统，也是光性蓝光灵敏度系统。尽管这些敏感性系统通常位于独立的光感受器中，但这两个不同的光感应系统会在Phy3分子中坐标并产生协同效应，因此赋予PHY3的增强的光敏感性。

项目成果

A single chromoprotein with triple chromophores acts as both a phytochrome and a phototropin

• DOI: 10.1073/pnas.0603569103
• 发表时间: 2006-11
• 期刊: Proceedings of the National Academy of Sciences
• 作者: T. Kanegae;Emi Hayashida;Chihiro Kuramoto;M. Wada

Photomorphogenesis in Plants and Bacteria : Function and signaltransduction mechanisms, 3rd ed.

植物和细菌中的光形态发生：功能和信号转导机制，第三版。

• 发表时间: 2006
• 作者: Endo;D;Murakami;S;Akazome;Y;Park MK.;Takeshi Kanegae;Takeshi Kanegae;Takeshi Kanegae
• 通讯作者: Takeshi Kanegae