Functional analyses of fern photoreceptor PHY3

蕨类植物光感受器 PHY3 的功能分析

• 批准号: 17570041
• 负责人: KANEGAE Takeshi
• 金额: $ 2.24万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570041/
• 关键词: plant; development; differentiation; photoreceptor; signal transduction; environmental response; フィトクロム; フォトトロピン

Phytochrome 3 (PHY3) is a red-light receptor found in some ferns, which preferentially grow under weak light conditions. Based on its predicted domain organization, fern PHY3 looks like a chimeric protein consists of both red・and blue-light photoreceptors. N-terminal half of PHY3 contains a chromophore-binding domain of phytochrome and its C-terminal half shows a remarkable similarity to phototropin, containing two LOV domains and a Ser/Thr kinase domain. To study functions of PHY3 polypeptide, we introduced CaMV 35S promoter driven Adiantum PHY3 gene into Arabidopsis phot1-5 phot2-1 plant and observed the phototropic responses. Here we report that PHY3 acts as a dual-channel photoreceptor with both the red-light sensing system of phytochrome and the blue-light sensing system of phototropin. Although these sensing-systems usually reside in independent photoreceptors, these two different light-sensing systems cooperate and generate a synergistic effect within PHY3 molecule, and consequently confer the enhanced light-sensitivity on PHY3.

光敏色素3 （PHY3）是在一些蕨类植物中发现的一种红光受体，它们优先在弱光条件下生长。根据预测的结构域组织，蕨类植物PHY3看起来像一个由红光和蓝光光感受器组成的嵌合蛋白。PHY3的n端半部分包含光敏色素的一个发色团结合结构域，其c端半部分与趋光蛋白非常相似，包含两个LOV结构域和一个Ser/Thr激酶结构域。为了研究PHY3多肽的功能，我们将CaMV 35S启动子驱动的Adiantum PHY3基因导入拟南芥phot1-5 - phot2-1植株，观察其趋光反应。本文报道了PHY3作为一种双通道光感受器，具有光敏色素的红光感应系统和光促素的蓝光感应系统。虽然这些传感系统通常存在于独立的光感受器中，但这两个不同的光传感系统在PHY3分子内相互合作并产生协同效应，从而赋予PHY3增强的光敏性。

项目成果

A single chromoprotein with triple chromophores acts as both a phytochrome and a phototropin

• DOI: 10.1073/pnas.0603569103
• 发表时间: 2006-11
• 期刊: Proceedings of the National Academy of Sciences
• 作者: T. Kanegae;Emi Hayashida;Chihiro Kuramoto;M. Wada

Photomorphogenesis in Plants and Bacteria : Function and signaltransduction mechanisms, 3rd ed.

植物和细菌中的光形态发生：功能和信号转导机制，第三版。

• 发表时间: 2006
• 作者: Endo;D;Murakami;S;Akazome;Y;Park MK.;Takeshi Kanegae;Takeshi Kanegae;Takeshi Kanegae
• 通讯作者: Takeshi Kanegae