Artificial-induced sex reversal and paradoxical sex reversal in medaka

青鳉人工诱发的性逆转和矛盾性逆转

• 批准号: 23570073
• 负责人: SHIBATA Naoki
• 金额: $ 3.41万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570073/
• 关键词: 性転換; 性ステロイド投与; メダカ; 逆説的性転換; 近交系; 系統差

Using six medaka inbred strains, their sex reversal process after administration of sex steroids to embryos were investigated. After estrogen administration, XY females were appeared in 5 strains, but the sensitivity and/or degree of sex-reversal were different among strains. Administration of androgen to XX specimen caused female to male sex reversal in various degree. On the other hand, male to female sex reversal in XY specimens termed paradoxical sex reversal were also observed at high dose of androgen in 4 strains. Thus the sex reversal process were highly varied among strains. In most strains, gonads showed strong atrophy after androgen administration. After hatching when clearing the exogenous androgen, these gonads were eventually recovered but their growth and the sexual differentiation were remarkably delayed.

以6个青鳉近交系为材料，研究了性类固醇激素处理青鳉胚胎后的性逆转过程。雌激素处理后，有5个菌株出现XY雌性，但不同菌株对性反转的敏感性和/或程度不同。 给XX标本注射雄激素可引起不同程度的性逆转。 另一方面，在高剂量雄激素作用下，4个品系的XY标本也出现了雄性向雌性的性反转，称为反常性反转。 因此，性逆转过程在菌株之间差异很大。 在大多数品系中，雄激素给药后性腺表现出强烈的萎缩。 孵化后，当清除外源雄激素时，这些性腺最终恢复，但它们的生长和性分化显着延迟。