Development of basic glycotechnology for construction of multifunctional proteoglycan molecules.

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Hydrolysis and transglycosylation reactions of hyaluronidase (from bovine testes) and cellulase (from A. nigar) were investigated with the aim of applying them in glycotechnology for proteoglycans. As reaction substrates, commercially available sugar chains in addition to proteoglycans, as well as oligosaccharides having linkage structures between a core peptide and a sugar chain of proteoglycan, and de-sulfated sugar chains that we prepared, were used. We found a sugar cahin structure that was degraded by hyaluronidase but possibly did not act as a donor substrate for the transglycosylation reaction. The optimal condition for proteoglycan hydrolysis by cellulase was determined, however, transglycosylation reaction could not be detected.