Functional studies based on the structure of brain water channel, aquaporin-4

基于脑水通道结构、aquaporin-4 的功能研究

• 批准号: 23570191
• 负责人: HIROAKI Yoko
• 金额: $ 3.41万
• 依托单位: Nagoya University (2012-2013)Kyoto University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570191/
• 关键词: 膜蛋白質; アクアポリン-４; 電子線結晶構造解析; アクアポリン-4; アクアポリン－４

AQP4 revealed in the brain at predominance is highly preserved between species. In order to understand the physiological function of AQP4 based on structural analysis, the astrocyte from mice including genetically engineered mice expressing AQP4 without adhesive function were comprehensively analyzed on mRNA as well as a protein level. The culture procedure was established that 90% of the cultured cells showed positive reaction to the astrocyte-markers, such as GFAP. Microarray analyses showed reduction of various kinases but increase of adhesion molecules in the genetically engineered mice compared with those in WT ones. A cytoskeleton related factors and integrin family proteins were found to be decreased by mass spectrometry.Because of devotion duty caused by my new affiliation, this project has to regrettably be stopped at the middle of the last fiscal year, while this project is interesting.

AQP4在大脑中显示的优势在物种之间被高度保存。为了在结构分析的基础上了解AQP4的生理功能，我们从mRNA和蛋白水平对表达AQP4无粘附功能的转基因小鼠及小鼠的星形胶质细胞进行了综合分析。建立培养程序，90%的培养细胞对星形胶质细胞标记物（如GFAP）有阳性反应。微阵列分析显示，与野生型小鼠相比，基因工程小鼠的各种激酶减少，但粘附分子增加。质谱分析发现细胞骨架相关因子和整合素家族蛋白减少。由于我的新单位带来的奉献责任，很遗憾这个项目不得不在上个财政年度中期停止，而这个项目是有趣的。

项目成果

Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish

小龙虾外侧巨纤维间隙连接的电子断层扫描分析

• DOI: 10.1016/j.jsb.2011.04.002
• 发表时间: 2011
• 期刊: J. Struct. Biol.
• 作者: Ohta;Y.;Nishikawa;K.;Hiroaki;Y. and Fujiyoshi;Y.
• 通讯作者: Y.

細胞生理学研究センター

细胞生理学研究中心

Two alternative conformations of a voltage-gated sodium channel

电压门控钠通道的两种替代构象

• 发表时间: 2013
• 期刊: J. Mol. Biol
• 作者: C. J. Tsai;K. Tani;K. Irie;Y. Hiroaki;T. Shimomura;D. G. McMillan;G. M. Cook;G. F.X. Schertler;Y. Fujiyoshi and X. D. Li
• 通讯作者: Y. Fujiyoshi and X. D. Li