Functional studies based on the structure of brain water channel, aquaporin-4

AQP4在大脑中的优势是高度保存的物种之间。为了了解基于结构分析的AQP4的生理功能，从mRNA以及蛋白质水平全面分析了来自小鼠的星形胶质细胞，包括表达无粘附功能的AQP4的基因工程小鼠。建立了90%的培养细胞对星形胶质细胞标志物如GFAP呈阳性反应的培养程序。基因芯片分析显示，与野生型小鼠相比，基因工程小鼠的各种激酶减少，但粘附分子增加。通过质谱分析发现细胞骨架相关因子和整合素家族蛋白质减少。由于我新加入的奉献职责，这个项目不得不遗憾地在上一个财政年度中期停止，而这个项目很有趣。

AQP4 revealed in the brain at predominance is highly preserved between species. In order to understand the physiological function of AQP4 based on structural analysis, the astrocyte from mice including genetically engineered mice expressing AQP4 without adhesive function were comprehensively analyzed on mRNA as well as a protein level. The culture procedure was established that 90% of the cultured cells showed positive reaction to the astrocyte-markers, such as GFAP. Microarray analyses showed reduction of various kinases but increase of adhesion molecules in the genetically engineered mice compared with those in WT ones. A cytoskeleton related factors and integrin family proteins were found to be decreased by mass spectrometry.Because of devotion duty caused by my new affiliation, this project has to regrettably be stopped at the middle of the last fiscal year, while this project is interesting.