Regulation of nuclear receptors by SUMO through recruitment of chromatin remodeling factors

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For understanding on the molecular processes of development, cell growth and homeostasis, it is required to reveal a regulation mechanism of binding of the transcription factors to the target genes. One of a useful method to introduce a new insight into the complex formation with the target DNA is to visualize the processes using the cell line that possesses a tandem repeat array of the mouse mammary tumor virus (MMTV) promoter that is the target of the steroid receptor (GR, glucocorticoid receptor). Using this system, we succeeded in finding of a novel complex formation on the MMTV array. Although the chromatin-remodeling factor, ARIP4, is localized on nuclear structure without the glucocorticoid, after addition of the ligand, ARIP4 is co-localized with GR on the target DNA. This suggests that the MMTV array system is useful to analyze an important role of nuclear structure in the regulation of nuclear transcriptional complex formation.