Analysis of interaction that facilitated thermo-adaptive evolution of E. coli

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Interaction facilitating growth at high temperature was analyzed by identify of interactor, transcriptome and mutation function analyses. We have identified lactic acid as candidate for major interactor, but we have found that lactic acid production pathway was not activated significantly during thermo-adaptive evolution by transcriptome analyses, and lactate permease gene, lldP, was mutated in late 45 degree adaptation period when interaction diminished.Six mutations fixed during early 45 degree adaptation were analyzed by estimating contribution to fitness at 45 degree. We found candidate mutations important for interaction appearance and disappearance. These candidate mutations were related to cellular protein stability. This study indicated that interaction facilitating growth at high temperature was achieved by combination of interactor and intracellular protein stability.