Positional cloning and functional analysis of CR-genes in Brassica oleracea

甘蓝CR基因的定位克隆及功能分析

• 批准号: 23580003
• 负责人: OKAZAKI KEIICHI
• 金额: $ 3.49万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580003/
• 关键词: 根こぶ病; Brassica oleracea; 病害抵抗性遺伝子; 遺伝分析; アブラナ科作物

In order to isolate clubroot resistance gene (CR-gene) in cabbage, we mapped 30 markers that can detected polymorphism in CR-gene regions of the susceptible and resistance parents. The recombinant plants, which had crossovers in the CR-gene region, were genotyped using our designed markers to obtain graph genotypes. The inoculation to the graph genotypes revealed that the CR-gene region is located around the KBr marker. We determined the DNA sequence of the CR-gene region from next-generation sequencing analysis of the susceptible parent resistance. The candidate gene region will be sub-cloned from BAC and cosmid clones, already cloned and contained the target region.

为了分离甘蓝根肿病抗性基因，在感病和抗病亲本的根肿病抗性基因区域分别定位了30个多态性标记。利用我们设计的标记对在CR基因区域具有交叉的重组植物进行基因分型，以获得图谱基因型。接种到图基因型揭示了CR基因区域位于KBr标记周围。我们通过对感病亲本抗性的下一代测序分析确定了CR基因区域的DNA序列。候选基因区域将从BAC和粘粒克隆中亚克隆，其已经克隆并含有靶区域。

项目成果

Recent progress of clubroot resistance breeding through marker-assisted selection in Brassica oleracea and B. rapa

甘蓝和白菜标记辅助育种抗根肿病研究进展

• 发表时间: 2012
• 作者: Md.Asad-ud-doullah;H Tomita;M Shimizu;S Matsumoto; R Fujimoto and K Okazaki
• 通讯作者: R Fujimoto and K Okazaki

Recent progress of clubroot resistance breeding through marker-assisted selection in Brassica oleracea and B,rapa

甘蓝、白菜标记辅助育种抗根肿病研究进展

• 发表时间: 2012
• 作者: MD.ASAD-UD-DOULLAH;H. TOMITA;M. SHIMIZU;MATSUMOTO;R. FUJIMOTO AND K. OKAZAKI
• 通讯作者: R. FUJIMOTO AND K. OKAZAKI

Towards cloning a clubroot resistance minor gene PbBo(Anju)4 in Brassica oleracea

甘蓝抗根肿病小基因PbBo(Anju)4的克隆

• 发表时间: 2012
• 作者: OMITA;M. SHIMIZU;M. A. UD-DOLLAH;R. FUJIMOTO and K. OKAZAKI
• 通讯作者: R. FUJIMOTO and K. OKAZAKI

Brassica oleracea根こぶ病抵抗性遺伝子の集積による抵抗性育種

积累甘蓝根肿病抗性基因的抗性育种

• 发表时间: 2012
• 作者: 花神彩香;赤神慶子;岸本利彦;四方哲也;鈴木伶実・藤戸聡史・星野洋一郎・小野寺康之;富田寛也・清水元樹・藤本龍・U. Doullah MA・松本哲・岡崎桂一
• 通讯作者: 富田寛也・清水元樹・藤本龍・U. Doullah MA・松本哲・岡崎桂一

Accumulation of quantitative trait loci conferring broad-spectrum clubroot resistance in Brassica oleracea

• DOI: 10.1007/s11032-013-9918-9
• 发表时间: 2013-12-01
• 期刊: MOLECULAR BREEDING
• 影响因子: 3.1
• 作者: Tomita, Hiroya;Shimizu, Motoki;Okazaki, Keiichi
• 通讯作者: Okazaki, Keiichi