Positional cloning and functional analysis of CR-genes in Brassica oleracea

为了分离甘蓝抗根瘤病基因(CR基因)，我们在甘蓝感病亲本和抗病亲本的CR基因区域定位了30个能检测到多态性的标记。利用我们设计的标记对在CR基因区有交叉的重组植株进行了基因分型，得到了图形基因型。对图型的接种表明，CR基因区位于KBR标记附近。我们通过对感病亲本的下一代测序分析，确定了CR基因区域的DNA序列。候选基因区域将从已经克隆的BAC和COSMID克隆中亚克隆，并包含靶区。

In order to isolate clubroot resistance gene (CR-gene) in cabbage, we mapped 30 markers that can detected polymorphism in CR-gene regions of the susceptible and resistance parents. The recombinant plants, which had crossovers in the CR-gene region, were genotyped using our designed markers to obtain graph genotypes. The inoculation to the graph genotypes revealed that the CR-gene region is located around the KBr marker. We determined the DNA sequence of the CR-gene region from next-generation sequencing analysis of the susceptible parent resistance. The candidate gene region will be sub-cloned from BAC and cosmid clones, already cloned and contained the target region.