Positional cloning and functional analysis of CR-genes in Brassica oleracea

为了分离甘蓝根肿病抗性基因，在感病和抗病亲本的根肿病抗性基因区域分别定位了30个多态性标记。利用我们设计的标记对在CR基因区域具有交叉的重组植物进行基因分型，以获得图谱基因型。接种到图基因型揭示了CR基因区域位于KBr标记周围。我们通过对感病亲本抗性的下一代测序分析确定了CR基因区域的DNA序列。候选基因区域将从BAC和粘粒克隆中亚克隆，其已经克隆并含有靶区域。

In order to isolate clubroot resistance gene (CR-gene) in cabbage, we mapped 30 markers that can detected polymorphism in CR-gene regions of the susceptible and resistance parents. The recombinant plants, which had crossovers in the CR-gene region, were genotyped using our designed markers to obtain graph genotypes. The inoculation to the graph genotypes revealed that the CR-gene region is located around the KBr marker. We determined the DNA sequence of the CR-gene region from next-generation sequencing analysis of the susceptible parent resistance. The candidate gene region will be sub-cloned from BAC and cosmid clones, already cloned and contained the target region.