Genetic analysis of root traits in Lotus corniculatus produced by gain-of-function strategy

本研究的目的是寻找能增加豆科植物生物量、根伸长和分化、提高根瘤菌产量的有用基因。在这里，拟南芥的cDNA被随机地转化到莲花中。利用FOX系统这一快速有效地挖掘有用性状的分子工具，对过表达拟南芥导入基因的植株进行了评价。我们测量了植株的上部和下部，以及根瘤菌在根部的产生率。通过这种方法，我们发现了基于形态特征表现出显著差异的植物品系，包括根瘤菌产量。从这些结果中收集的所有信息都汇编在一个正常工作的数据库中。特别是，该数据库将为研究用途而准备和开放。这一结果对甘氨酸的分子育种和提高甘氨酸产量具有一定的参考价值。

The objective of this study was to investigate useful genes that can increase plant biomass, root elongation and differentiation, and increase rhizobium production rates in legumes. Here, Arabidopsis thaliana cDNAs were randomly transformed into Lotus corniculatus. Using FOX system, a molecular tool to rapidly and efficiently dig useful trait, plants that had overexpressed the introduced genes from Arabidopsis were evaluated. We measured the upper parts and lower parts of the plant, and also the production rates of rhizobia in the roots. From this method, we found plant lines that showed significant differentiations based on the morphological characteristics, including rhizobia production. All information gathered from these results was compiled in a working database. Specially, this database will be prepared and opened for research use. Furthermore, this result is useful for the molecular breeding of glycine to improve glycine production.