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Genetic analysis of root traits in Lotus corniculatus produced by gain-of-function strategy

功能获得策略产生的百脉根根性状的遗传分析

基本信息

批准号：
23580010
负责人：
AKASHI Ryo
金额：
$ 3.41万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

The objective of this study was to investigate useful genes that can increase plant biomass, root elongation and differentiation, and increase rhizobium production rates in legumes. Here, Arabidopsis thaliana cDNAs were randomly transformed into Lotus corniculatus. Using FOX system, a molecular tool to rapidly and efficiently dig useful trait, plants that had overexpressed the introduced genes from Arabidopsis were evaluated. We measured the upper parts and lower parts of the plant, and also the production rates of rhizobia in the roots. From this method, we found plant lines that showed significant differentiations based on the morphological characteristics, including rhizobia production. All information gathered from these results was compiled in a working database. Specially, this database will be prepared and opened for research use. Furthermore, this result is useful for the molecular breeding of glycine to improve glycine production.

本研究的目的是寻找能增加豆科植物生物量、根系伸长和分化、提高根瘤菌产量的有用基因。在这里，拟南芥的cdna被随机转化为莲花。利用快速高效挖掘有用性状的分子工具FOX系统，对拟南芥引进基因过表达的植株进行了评价。我们测量了植株的上部和下部，以及根系中根瘤菌的产量。通过这种方法，我们发现植物系在形态特征上表现出显著的分化，包括根瘤菌的产生。从这些结果中收集的所有信息都汇编在一个工作数据库中。特别地，这个数据库将准备和开放供研究使用。此外，该结果可为甘氨酸分子育种提高甘氨酸产量提供参考。